Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany.
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Feb 1;883-884:141-6. doi: 10.1016/j.jchromb.2011.10.037. Epub 2011 Nov 3.
Bioactive glycerophospho- and sphingolipids species are involved in the regulation of numerous biological processes and implicated in the pathophysiology of various diseases. Here we review electrospray ionization tandem mass spectrometric (ESI-MS/MS) methods for the analysis of these bioactive lipid species in blood including lysophosphatidic acid (LPA), lysophosphatidylcholine (LPC), bis(monoacylglycero)phosphate (BMP), ceramide (Cer), sphingosine-1-phosphate (S1P) and sphingosylphosphorylcholine (SPC). Beside direct tandem mass spectrometric and liquid chromatography coupled approaches, we present an overview of concentrations of these bioactive lipids in plasma. The analytical strategies are discussed together with aspects of sample preparation, quantification and sample stability.
生物活性甘油磷酯和神经鞘脂类物质参与了许多生物过程的调节，并与各种疾病的病理生理学有关。在这里，我们综述了用于分析血液中这些生物活性脂质物质的电喷雾串联质谱（ESI-MS/MS）方法，包括溶血磷脂酸（LPA）、溶血磷脂酰胆碱（LPC）、双（单酰基甘油）磷酸（BMP）、神经酰胺（Cer）、鞘氨醇-1-磷酸（S1P）和鞘氨醇磷酸胆碱（SPC）。除了直接串联质谱和液相色谱耦联方法外，我们还介绍了这些生物活性脂质在血浆中的浓度概述。本文还讨论了分析策略以及样品制备、定量和样品稳定性等方面。
