Liebisch Gerhard, Schmitz Gerd
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany.
Methods Mol Biol. 2009;580:29-37. doi: 10.1007/978-1-60761-325-1_3.
Lysophosphatidylcholine (LPC) is a bioactive lipid implicated to play a functional role in various diseases including atherosclerosis, diabetes, cancer, and inflammation. Conventional methods are of limited value for a systematic evaluation of LPC species concentrations due to complicated, time-consuming procedures. Here we describe a methodology based on electrospray ionization tandem mass spectrometry (ESI-MS/MS) applicable for high-throughput LPC species quantification. This assay provides accuracy and precision sufficient for the analysis of large clinical studies as well as basic biochemical studies in a broad range of biological material including plasma, tissues, and cell culture material. This method may be combined with methods based on the same analytical setup for glycerophospholipid, sphingolipid, and sterol analysis to evaluate LPC species in relation to other lipid species profiles.
溶血磷脂酰胆碱（LPC）是一种生物活性脂质，被认为在包括动脉粥样硬化、糖尿病、癌症和炎症在内的各种疾病中发挥功能性作用。由于传统方法操作复杂、耗时，对于LPC种类浓度的系统评估价值有限。在此，我们描述了一种基于电喷雾电离串联质谱（ESI-MS/MS）的方法，适用于高通量LPC种类定量。该检测方法提供的准确度和精密度足以用于大型临床研究以及对包括血浆、组织和细胞培养材料在内的广泛生物材料进行基础生物化学研究。该方法可与基于相同分析设置的甘油磷脂、鞘脂和固醇分析方法相结合，以评估LPC种类与其他脂质种类谱的关系。
