Institut für Technische und Makromolekulare Chemie (ITMC), RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany.
Bioresour Technol. 2012 Jan;104:799-802. doi: 10.1016/j.biortech.2011.10.071. Epub 2011 Nov 6.
This communication explores the use of seawater (1X) and concentrated seawater (2X and 4X) as reaction media for the enzyme-catalyzed depolymerization of cellulose. The commercially available Accellerase-1500® - a "cocktail" of different glycosidases - is able to depolymerize several amorphous celluloses and microcrystalline cellulose Avicel® in these reaction media, at slightly lower rates (ca. 90%) than those observed when reactions are performed in pure citrate buffer (control reactions). Remarkably, at concentrated seawater effluents enzymes also display significant rates of cellulose hydrolysis. Considering the expected increasing shortages in accessibility to fresh drinkable water, the herein-reported concept may provide novel inspiring leads for a smart use of resources in an environmentally-friendly and efficient manner, and for the genetic development of cellulases highly active and stable in concentrated seawater solutions.
本通讯探讨了使用海水（1X）和浓缩海水（2X 和 4X）作为酶促纤维素解聚的反应介质。市售的 Accellerase-1500® - 一种不同糖苷酶的“混合物” - 能够在这些反应介质中解聚几种无定形纤维素和微晶纤维素 Avicel®，其速率略低（约 90%）与在纯柠檬酸盐缓冲液（对照反应）中观察到的反应相比。值得注意的是，在浓缩海水中，酶也显示出显著的纤维素水解速率。考虑到新鲜饮用水可及性预计会增加短缺，本报告中的概念可能为以环保和高效的方式明智地利用资源以及在浓缩海水中具有高活性和稳定性的纤维素酶的遗传开发提供新的启示。
