College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
Bioresour Technol. 2010 Sep;101(17):6685-91. doi: 10.1016/j.biortech.2010.03.085. Epub 2010 Apr 10.
The effects of additives on hydrolysis of microcrystalline cellulose (Avicel PH101) were examined using commercial cellulose-degrading enzymes (Celluclast 1.5L and Novozyme 188). Polyethylene glycol 4000 (PEG4000) was the most effective additive tested. When PEG4000 was added at 0.05 g/g glucan, the conversion of Avicel PH101 increased 91% (from 41.1% to 78.9%). The cellulase activity of Celluclast 1.5L increased 27.5% with PEG4000 addition. A positive effect on enzyme stabilities of Celluclast 1.5L and Novozyme 188 also occurred with PEG4000 addition. During hydrolysis process, significant changes in free protein concentration and cellulase activity were observed on Avicel PH101. More than 90% of the original enzyme activity remained in the solution after 48 h hydrolysis. Thus, PEG4000 addition is an efficient method to enhance digestibility of cellulosic materials and make enzyme recovery possible and valuable. This provides an opportunity of decreasing the operational cost of the hydrolysis process.
研究了添加剂对微晶纤维素（Avicel PH101）水解的影响，使用了商业纤维素降解酶（Celluclast 1.5L 和 Novozyme 188）。聚乙二醇 4000（PEG4000）是测试过的最有效的添加剂。当 PEG4000 以 0.05 g/g 葡聚糖的量添加时，Avicel PH101 的转化率增加了 91%（从 41.1%增加到 78.9%）。添加 PEG4000 使 Celluclast 1.5L 的纤维素酶活性增加了 27.5%。添加 PEG4000 对 Celluclast 1.5L 和 Novozyme 188 的酶稳定性也有积极影响。在水解过程中，在 Avicel PH101 上观察到游离蛋白浓度和纤维素酶活性的显著变化。在 48 小时水解后，超过 90%的原始酶活性仍保留在溶液中。因此，添加 PEG4000 是一种有效提高纤维素材料消化率并使酶回收成为可能和有价值的方法。这为降低水解过程的运营成本提供了机会。
