Instituto de Química, Universidad Nacional Autónoma de México, Coyoacán, México, D.F. 04510, Mexico.
J Ethnopharmacol. 2012 Jan 6;139(1):164-70. doi: 10.1016/j.jep.2011.10.033. Epub 2011 Nov 9.
Several species of Annona (Annonaceae) are used in traditional Mexican medicine by their anti-anxiety, anticonvulsant and tranquilizing properties. It has been reported that the alkaloids isolated from some species of the Annona have affinity to serotonergic 5-HT(1A) receptors and modulate dopaminergic transmission, which is involved in depressive disorders.
To investigate the antidepressant-like effect of an alkaloid extract from the aerial parts of Annona cherimola (TA) in mice.
The antidepressant-like effect was evaluated in the forced swimming test. To elucidate a possible mechanism of action, experiments of synergism with antidepressant drugs, such as imipramine (IMI), clomipramine (CLIMI), and fluoxetine (FLX), were carried out. The neurotransmitter content (DA: dopamine, 5HT: serotonin and its metabolites, HVA: homovanillic acid and 5HIAA: 5-hydroxyindoleacetic) in the whole brain of mice were also determined by HPLC method. TA chemical composition was determined using high performance liquid chromatography-electrospray mass spectrometry.
The results showed that repeated treatment with TA produced antidepressant-like effects in mice. This effect was not related to an increase in locomotor activity. Administration of TA facilitated the antidepressant effect of IMI and CLIMI as well as increased the turnover of DA and 5-HT. The alkaloids: 1,2-dimethoxy-5,6,6a,7-tetrahydro-4H-dibenzoquinoline-3,8,9,10-tetraol, anonaine, liriodenine, and nornuciferine were the main constituents of TA.
Results showed that TA produces an antidepressant-like action from a generalized increase in monominergic turnover, supporting the use in tradicional medicine of Annona cherimolia, and strongly suggest its therapeutic potency as an antidepressant agent.
几种番荔枝属（番荔枝科）植物因其具有抗焦虑、抗惊厥和镇静作用，被用于传统的墨西哥医学。据报道，从某些番荔枝属植物中分离得到的生物碱对 5-羟色胺能 5-HT(1A)受体具有亲和力，并调节多巴胺能传递，这与抑郁障碍有关。
研究番荔枝（TA）地上部分生物碱提取物对小鼠的抗抑郁样作用。
采用强迫游泳试验评价抗抑郁样作用。为阐明可能的作用机制，进行了与抗抑郁药物（如丙咪嗪（IMI）、氯米帕明（CLIMI）和氟西汀（FLX））协同作用的实验。还通过 HPLC 法测定了小鼠全脑神经递质含量（DA：多巴胺，5HT：血清素及其代谢物，HVA：高香草酸和 5HIAA：5-羟吲哚乙酸）。采用高效液相色谱-电喷雾质谱联用（HPLC-ESI-MS）法测定 TA 的化学成分。
结果表明，重复给予 TA 可产生抗抑郁样作用。这种作用与运动活性的增加无关。TA 给药促进了 IMI 和 CLIMI 的抗抑郁作用，并增加了 DA 和 5-HT 的周转率。生物碱：1,2-二甲氧基-5,6,6a,7-四氢-4H-二苯并喹啉-3,8,9,10-四醇、阿农碱、利鲁定和去甲乌药碱是 TA 的主要成分。
结果表明，TA 通过单胺能传递的普遍增加产生抗抑郁样作用，支持传统医学中使用番荔枝，强烈提示其作为抗抑郁剂的治疗潜力。
