School of Mechanical and Aerospace Engineering, Seoul National University, Seoul 151-742, Korea.
Nanotechnology. 2011 Dec 9;22(49):494017. doi: 10.1088/0957-4484/22/49/494017. Epub 2011 Nov 21.
We present the effect of nanotopographically defined surfaces on adipocyte differentiation using various nanogroove patterns. Parallel nanogroove arrays with equal inter-groove distance (400, 550, 800 nm width) and varying distances (550 nm width with three different spacings of 550, 1100, and 2750 nm) were fabricated by UV-assisted capillary force lithography (CFL) on 18 mm diameter glass coverslips using biocompatible polyurethane (PU)-based material. After coating with fibronectin and subsequent culture of 3T3-L1 preadipocytes, the degree of adipocyte differentiation was determined by Oil Red O staining and adipogenic gene expression. We observed that adipocyte differentiation was slightly but substantially affected by culture on various nanogrooved surfaces. In particular, the cell crawling into nanogrooves contributed substantially to an enhanced level of differentiation with higher contact guidance, suggesting that cell-to-surface interactions would play a role for the adipocyte differentiation.
我们使用各种纳米凹槽图案展示了纳米形貌定义的表面对脂肪细胞分化的影响。通过紫外辅助毛细作用力光刻（CFL）在 18mm 直径的玻璃盖玻片上用生物相容性的基于聚氨酯（PU）的材料制备具有相等的凹槽间距离（400nm、550nm 和 800nm 宽）和不同间距（550nm 宽，间距分别为 550nm、1100nm 和 2750nm 的三种不同间隔）的平行纳米凹槽阵列。在用纤维连接蛋白涂覆后并在 3T3-L1 前体脂肪细胞培养之后，通过油红 O 染色和脂肪生成基因表达来确定脂肪细胞分化的程度。我们观察到，在各种纳米凹槽表面上培养对脂肪细胞分化有轻微但显著的影响。特别是，细胞爬入纳米凹槽中对更高的接触引导有显著的促进作用，表明细胞与表面的相互作用将对脂肪细胞分化起作用。
