大西洋鲑（Salmo salar L.）肝细胞中脂肪酸及对生物活性化合物的基因表达反应
Fatty acids and gene expression responses to bioactive compounds in Atlantic salmon (Salmo salar L.) hepatocytes.
作者信息
Schiller Vestergren Anna Lotta, Trattner Sofia, Mráz Jan, Ruyter Bente, Pickova Jana
机构信息
Department of Food Science, Swedish University of Agricultural Sciences, Uppsala, Sweden.
出版信息
Neuro Endocrinol Lett. 2011;32 Suppl 2:41-50.
OBJECTIVES
Effects of bioactive compounds on expression of genes involved in lipid metabolism and fatty acid composition were investigated in Atlantic salmon (Salmo salar L.) primary hepatocytes.
METHODS
Five treatments were investigated: I) genistein 0.005 mM, II) genistein 0.025 mM, III) lipoic acid 0.2 mM, IV) sesamin/episesamin 0.05 mM, V) sesamin 0.05 mM and compared to controls. The relative expression of genes involved in lipid homeostasis was analysed after 12h and 48h.
RESULTS
Incubation with lipoic acid, sesamin and episesamin/sesamin for 48h had significant effect on all analysed genes involved in lipid uptake, β-oxidation, elongation and desaturation, some effects were detected on the expression of peroxisome prolifertor-activated receptor (PPARs). Also effects on the fatty acid composition were found.
CONCLUSION
The strongest effect of bioactive copounds on hepatocyte gene expression was detected after 48 hours.
目的
在大西洋鲑（Salmo salar L.）原代肝细胞中研究生物活性化合物对参与脂质代谢的基因表达及脂肪酸组成的影响。
方法
研究了五种处理方式：I）0.005 mM 染料木黄酮，II）0.025 mM 染料木黄酮，III）0.2 mM 硫辛酸，IV）0.05 mM 芝麻素/表芝麻素，V）0.05 mM 芝麻素，并与对照组进行比较。在12小时和48小时后分析参与脂质稳态的基因的相对表达。
结果
用硫辛酸、芝麻素和表芝麻素/芝麻素孵育48小时对所有参与脂质摄取、β-氧化、延长和去饱和的分析基因有显著影响，对过氧化物酶体增殖物激活受体（PPARs）的表达也有一些影响。还发现了对脂肪酸组成的影响。
结论
48小时后检测到生物活性化合物对肝细胞基因表达的最强影响。
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