Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules and College of Chemistry and Chemical Engineering, Hubei University, Wuhan, P. R. China.
J Sep Sci. 2012 Jan;35(1):137-44. doi: 10.1002/jssc.201100767. Epub 2011 Nov 21.
A novel solid-phase microextraction (SPME) method based on molecularly imprinted polymer (MIP) monolith as the sorbent for the selective extraction of thiamphenicol (TAP) in milk and honey was developed. The newly developed MIP monolith was produced using TAP as the template molecule, 4-vinylpyridine (4-VP) as the functional monomer. The TAP-MIP monolith synthesized in a micropipette tip could be connected with syringes in different sizes simply to perform SPME process without any other treatment. The derivated MIP monolith showed high selectivity and enrichment ability for TAP. A simple, rapid and sensitive method for the determination of TAP in milk and honey using polymer monolith microextraction (PMME) based on the MIP monolith combined with high-performance liquid chromatography-photodiodes array detector was developed. Several parameters affecting MIP monolith microextraction were investigated, including the flow rate, volume, pH and salt concentration of sample, the type and volume of washing solution, the type and flow rate of eluent. The recovery of this method for TAP was investigated and high recoveries of 92.9-99.3% from milk and honey were obtained with relative standard deviations less than 4.9%.
建立了一种基于分子印迹聚合物（MIP）整体柱的新型固相微萃取（SPME）方法，用于选择性萃取牛奶和蜂蜜中的噻苯咪唑（TAP）。新开发的 MIP 整体柱以 TAP 为模板分子，4-乙烯基吡啶（4-VP）为功能单体合成。在微吸管尖端合成的 TAP-MIP 整体柱可以简单地与不同尺寸的注射器连接，无需任何其他处理即可进行 SPME 过程。衍生的 MIP 整体柱对 TAP 表现出高选择性和富集能力。建立了一种基于 MIP 整体柱的聚合物整体微萃取（PMME）结合高效液相色谱-光电二极管阵列检测器测定牛奶和蜂蜜中 TAP 的简单、快速、灵敏的方法。研究了影响 MIP 整体柱微萃取的几个参数，包括样品的流速、体积、pH 值和盐浓度、洗涤液的类型和体积、洗脱液的类型和流速。考察了该方法对 TAP 的回收率，从牛奶和蜂蜜中得到了 92.9-99.3%的高回收率，相对标准偏差小于 4.9%。
