Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Institute of Environmental Chemistry, College of Chemistry, Central China Normal University, Wuhan 430079, China.
Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Institute of Environmental Chemistry, College of Chemistry, Central China Normal University, Wuhan 430079, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Mar 1;951-952:104-9. doi: 10.1016/j.jchromb.2014.01.030. Epub 2014 Jan 29.
A pipette tip-based molecularly imprinted polymer monolith microextraction (PT-MIPMME) method was developed for the selective extraction of difenoconazole in tap water and grape juice. In this method, molecularly imprinted polymer (MIP) monolith used as the sorbent was synthesized at the tip of a micropipette. This in situ polymerization reaction used difenoconazole as the template and methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linker and the mixture of toluene-dodecanol as the porogenic solvent. The pipette tip containing MIP monolith was matched to a syringe for performing the polymer monolith microextraction (PMME). Several parameters affecting the proposed PT-MIPMME method were investigated, including the flow rate, sample volume, pH and salt concentration of sample, the type and volume of eluent. Under the optimal conditions, the PT-MIPMME method showed a low limit of detection of 0.5μgL(-1). The recoveries were in the range of 87.6-95.4% with relative standard deviations less than 4.9%. The results showed that difenoconazole was selectively enriched from tap water and grape juice samples.
基于移液器吸头的分子印迹聚合物整体微萃取（PT-MIPMME）方法被开发用于选择性萃取自来水中和葡萄汁中的烯唑醇。在该方法中，作为吸附剂的分子印迹聚合物（MIP）整体在微量移液器的尖端合成。该原位聚合反应以烯唑醇为模板，甲基丙烯酸（MAA）为功能单体，乙二醇二甲基丙烯酸酯（EGDMA）为交联剂，甲苯-十二醇混合物为致孔剂。含有 MIP 整体的移液器吸头与注射器匹配，用于进行聚合物整体微萃取（PMME）。研究了影响所提出的 PT-MIPMME 方法的几个参数，包括流速、样品体积、样品的 pH 值和盐浓度、洗脱剂的类型和体积。在最佳条件下，PT-MIPMME 方法的检测限低至 0.5μg/L。回收率在 87.6-95.4%范围内，相对标准偏差小于 4.9%。结果表明，烯唑醇可以从自来水中和葡萄汁样品中选择性富集。
