School of Physics and Astronomy, University of Southampton, Southampton, SO171BJ, UK.
Small. 2012 Jan 9;8(1):122-30. doi: 10.1002/smll.201101422. Epub 2011 Nov 18.
The interactions between noncancerous, primary endothelial cells and gold nanoparticles with different morphologies but the same ligand capping are investigated. The endothelial cells are incubated with gold nanospheres, nanorods, hollow gold spheres, and core/shell silica/gold nanocrystals, which are coated with monocarboxy (1-mercaptoundec-11-yl) hexaethylene glycol (OEG). Cell viability studies show that all types of gold particles are noncytotoxic. The number of particles taken up by the cells is estimated using inductively coupled plasma (ICP), and are found to differ depending on particle morphology. The above results are discussed with respect to heating efficiency. Using experimental data reported earlier and theoretical model calculations which take into account the physical properties and distribution of particles in the cellular microenvironment, it is found that collective heating effects of several cells loaded with nanoparticles must be included to explain the observed viability of the endothelial cells.
研究了具有不同形态但相同配体封端的非癌原发性内皮细胞与金纳米粒子之间的相互作用。将内皮细胞与金纳米球、纳米棒、中空金球和核/壳二氧化硅/金纳米晶孵育，这些金纳米粒子都被单羧基（1-巯基十一烷-11-基）六亚乙基二醇（OEG）覆盖。细胞活力研究表明，所有类型的金粒子均无细胞毒性。使用电感耦合等离子体（ICP）来估计细胞摄取的粒子数量，并发现其取决于粒子形态。根据加热效率对上述结果进行了讨论。使用早期报道的实验数据和理论模型计算，这些计算考虑了粒子在细胞微环境中的物理性质和分布，发现必须包括载有纳米粒子的几个细胞的集体加热效应，才能解释观察到的内皮细胞活力。
