Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, People's Republic of China.
Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge CB3 0AS, United Kingdom.
Chem Rev. 2022 Aug 10;122(15):12495-12543. doi: 10.1021/acs.chemrev.2c00050. Epub 2022 Jun 27.
Super-resolution imaging techniques that overcome the diffraction limit of light have gained wide popularity for visualizing cellular structures with nanometric resolution. Following the pace of hardware developments, the availability of new fluorescent probes with superior properties is becoming ever more important. In this context, fluorescent nanoparticles (NPs) have attracted increasing attention as bright and photostable probes that address many shortcomings of traditional fluorescent probes. The use of NPs for super-resolution imaging is a recent development and this provides the focus for the current review. We give an overview of different super-resolution methods and discuss their demands on the properties of fluorescent NPs. We then review in detail the features, strengths, and weaknesses of each NP class to support these applications and provide examples from their utilization in various biological systems. Moreover, we provide an outlook on the future of the field and opportunities in material science for the development of probes for multiplexed subcellular imaging with nanometric resolution.
超分辨率成像技术克服了光的衍射极限，为纳米分辨率可视化细胞结构提供了广泛的应用。随着硬件发展的步伐，具有更好性能的新型荧光探针的可用性变得越来越重要。在这种背景下，荧光纳米粒子（NPs）作为明亮且稳定的探针引起了越来越多的关注，它们解决了传统荧光探针的许多缺点。NPs 在超分辨率成像中的应用是一个新的发展方向，这也是当前综述的重点。我们概述了不同的超分辨率方法，并讨论了它们对荧光 NPs 性能的要求。然后，我们详细回顾了每一类 NPs 的特点、优势和不足，以支持这些应用，并提供了它们在各种生物系统中的应用实例。此外，我们还展望了该领域的未来，并探讨了在材料科学方面为发展纳米分辨率的多通道亚细胞成像探针提供的机会。
