Dass S B, Reddy C A
Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1101.
FEMS Microbiol Lett. 1990 Jun 1;57(3):221-4. doi: 10.1111/j.1574-6968.1990.tb04233.x.
Growth of Phanerochaete chrysosporium in a nitrogen-limited medium buffered with sodium acetate, instead of the commonly used 2,2-dimethylsuccinate (DMS), resulted in quantitative and qualitative differences in the production of various extracellular lignin peroxidases (LIPs) and manganese-dependent peroxidases (MNPs) involved in lignin degradation. The results indicate that production of LIPs and MNPs can be selectively enhanced by manipulation of culture conditions. Partial N-terminal analyses of the major LIPs and MNPs have made it possible to assign a specific protein to the specific genes and cDNAs that have been reported recently. The LIPs and MNPs differed widely in their ability to decolorize various dyes that are known to be degraded by the lignin degrading enzyme system of P. chrysosporium.
在以醋酸钠缓冲而非常用的2,2-二甲基琥珀酸（DMS）的氮限制培养基中，黄孢原毛平革菌的生长导致参与木质素降解的各种细胞外木质素过氧化物酶（LIPs）和锰依赖性过氧化物酶（MNPs）的产生在数量和质量上存在差异。结果表明，通过控制培养条件可以选择性地提高LIPs和MNPs的产量。对主要LIPs和MNPs的部分N端分析使得将特定蛋白质与最近报道的特定基因和cDNA进行对应成为可能。LIPs和MNPs在使各种已知可被黄孢原毛平革菌木质素降解酶系统降解的染料脱色的能力上有很大差异。
