Reddy C A
Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1101.
J Biotechnol. 1993 Jul;30(1):91-107. doi: 10.1016/0168-1656(93)90030-q.
The lignin-degrading white-rot fungus Phanerochaete chrysosporium produces two families of extracellular peroxidases designated lignin peroxidases (LIPs) and manganese-dependent peroxidases (MNPs) which are components of the lignin degradation system of this organism. The number and types of LIP and MNP isozymes produced vary dramatically in response to changes in culture conditions. Protease-mediated degradation of LIPs was shown to be the major cause for the decay of LIP activity in idiophasic cultures of P. chrysosporium. Use of biochemical mutants has not only yielded information on the relative importance of LIPs and MNPs in lignin degradation but has given us insights into the regulation of production of LIPs and MNPs. The genes encoding the major LIPs have been cloned and sequenced and were shown to have a high degree of homology to each other. Karyotyping studies indicated that heterokaryotic strains contain ten chromosomes and that the LIP genes are distributed on at least two chromosomes.
降解木质素的白腐真菌黄孢原毛平革菌产生两类胞外过氧化物酶，分别称为木质素过氧化物酶（LIPs）和锰依赖过氧化物酶（MNPs），它们是该生物体木质素降解系统的组成部分。LIP和MNP同工酶的数量和类型会随着培养条件的变化而显著不同。蛋白酶介导的LIPs降解被证明是黄孢原毛平革菌对数期培养物中LIP活性下降的主要原因。使用生化突变体不仅获得了关于LIPs和MNPs在木质素降解中相对重要性的信息，还让我们深入了解了LIPs和MNPs产生的调控机制。编码主要LIPs的基因已被克隆和测序，结果显示它们彼此之间具有高度同源性。核型分析研究表明，异核体菌株含有十条染色体，且LIP基因分布在至少两条染色体上。
