Owen R J, Costas M, Sloss L, Lastovica A, Le Roux E
National Collection of Type Cultures, Central Public Health Laboratory, London, U.K.
FEMS Microbiol Lett. 1990 Jun 1;57(3):329-35. doi: 10.1016/0378-1097(90)90089-9.
Twenty-eight isolates of catalase-negative/weak (CNW) thermophilic campylobacters from human blood and faecal cultures were characterized by one-dimensional (1-D) high-resolution SDS-PAGE of cellular proteins. A further 11 Campylobacter strains were included for reference purposes. Partial protein patterns were used as the basis for numerical analysis, which showed that all of the hippurate-positive strains had a high similarity to C. jejuni. Two subclusters were formed within C. jejuni corresponding to C. jejuni subsp. doylei (15 strains) and C. jejuni subsp. jejuni (4 strains). Most of the paediatric strains from South Africa were members of C. jejuni subsp. doylei. Hippurate-negative CNW thermophilic strains were identified as "C. upsaliensis". The analysis demonstrated that the catalase-negative C. jejuni strains were quite distinct from "C. upsaliensis" and that electrophoretic protein patterns provide an excellent criterion for the identification of subspecies within C. jejuni.
通过对细胞蛋白进行一维（1-D）高分辨率SDS-PAGE，对从人类血液和粪便培养物中分离出的28株过氧化氢酶阴性/弱阳性（CNW）嗜热弯曲杆菌进行了特征分析。另外纳入了11株弯曲杆菌菌株作为参考。部分蛋白质图谱用作数值分析的基础，结果表明所有马尿酸盐阳性菌株与空肠弯曲菌高度相似。在空肠弯曲菌内形成了两个亚群，分别对应空肠弯曲菌多氏亚种（15株）和空肠弯曲菌空肠亚种（4株）。来自南非的大多数儿科菌株是空肠弯曲菌多氏亚种的成员。马尿酸盐阴性的CNW嗜热菌株被鉴定为“乌普萨拉弯曲菌”。分析表明，过氧化氢酶阴性的空肠弯曲菌菌株与“乌普萨拉弯曲菌”截然不同，并且电泳蛋白质图谱为空肠弯曲菌内亚种的鉴定提供了极佳的标准。
