Owen R J, Costas M, Sloss L L
National Collection of Type Cultures, Central Public Health Laboratory, London, UK.
Eur J Epidemiol. 1988 Sep;4(3):277-83. doi: 10.1007/BF00148910.
Twenty-seven strains comprising 23 clinical isolates of nitrate negative campylobacters (NNC) from Australia, South Africa, the United Kingdom and the Federal Republic of Germany, a representative of the CNW (catalase negative/weak) group and reference strains of three other Campylobacter species, were characterized by one-dimensional SDS-polyacrylamide gel electrophoresis of cellular proteins. The protein patterns were highly reproducible, and were used as the basis for a numerical analysis which showed that the reference strain (NCTC 11951) of Campylobacter jejuni subspecies "doylei", and 20 NNC isolates formed a distinct group at the 74% similarity level. The protein patterns showed unexpectedly low similarity between subspecies "doylei" and the type strain of Campylobacter jejuni and revealed that some NNC strains were quite distinct from subspecies "doylei". Four electrophoretic (EP) types (I-IV) were identified from phenons formed at the 81% similarity level. Three of these (I, III, IV) corresponded to geographical location of strain isolation but the type II strains were from diverse locations. The correlation observed between EP-type, catalase production and sensitivity to 2, 3, 5, triphenyltetrazolium chloride indicated these latter two tests might be useful for biotyping within the subspecies.
对27株细菌进行了细胞蛋白的一维SDS聚丙烯酰胺凝胶电泳分析，这些细菌包括来自澳大利亚、南非、英国和德意志联邦共和国的23株硝酸盐阴性弯曲杆菌（NNC）临床分离株、一株CNW（过氧化氢酶阴性/弱阳性）组代表菌以及其他三种弯曲杆菌属的参考菌株。蛋白图谱具有高度可重复性，并作为数值分析的基础，该分析表明空肠弯曲菌“多伊亚种”的参考菌株（NCTC 11951）和20株NNC分离株在74%的相似性水平上形成一个独特的组。蛋白图谱显示“多伊亚种”与空肠弯曲菌模式菌株之间的相似性出人意料地低，并且表明一些NNC菌株与“多伊亚种”有很大不同。从81%相似性水平形成的类群中鉴定出四种电泳（EP）类型（I-IV）。其中三种（I、III、IV）与菌株分离的地理位置相对应，但II型菌株来自不同地点。观察到的EP类型、过氧化氢酶产生与对2,3,5-三苯基氯化四氮唑的敏感性之间的相关性表明，后两项试验可能有助于该亚种内的生物分型。
