ICORP Organ Regeneration Project, Japan Science and Technology Agency (JST), Tokyo 153-8902, Japan.
Dev Growth Differ. 2011 Dec;53(9):971-81. doi: 10.1111/j.1440-169X.2011.01304.x. Epub 2011 Nov 22.
We cloned the gene for the CXC-type chemokine ligand, Xenopus CXCLC (XCXCLC), the transcripts of which were detected at the dorsal midline during the gastrula and neurula stages. XCXCLC overexpression resulted in the attraction of nearby mesodermal cells, and the excess of chemoattractant interfered with convergent and extension movements. The direction of the deep neural plate cells around the notoplate was also controlled by XCXCLC. Fluorescence signals for XCXCLC + enhanced green fluorescent protein derivatives accumulated around the notochord region. These results indicate that XCXCLC attracts adjacent cells to the midline region, so as to ensure accurate lateral-medial directional tissue convergence during gastrulation and neurulation.
我们克隆了 CXC 型趋化因子配体的基因，即非洲爪蟾 CXCLC（XCXCLC），其转录本在原肠胚和神经胚阶段均在背中线检测到。XCXCLC 的过表达导致附近中胚层细胞的趋化作用，过多的趋化因子干扰了会聚延伸运动。XCXCLC 还控制着神经板细胞在顶板周围的方向。XCXCLC+增强型绿色荧光蛋白衍生物的荧光信号在脊索区域周围积累。这些结果表明，XCXCLC 将相邻细胞吸引到中线区域，从而确保在原肠胚和神经胚形成过程中准确地进行侧向到中侧方向的组织会聚。
