Goto Toshiyasu, Ito Yuzuru, Michiue Tatsuo
Department of Molecular Cell Biology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan.
Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
Dev Growth Differ. 2018 May;60(4):226-238. doi: 10.1111/dgd.12432. Epub 2018 Apr 26.
Several chemokine molecules control cell movements during early morphogenesis. However, it is unclear whether chemokine molecules affect cell fate. Here, we identified and characterized the CXC-type chemokine ligand in Xenopus laevis, Xenopus CXCLh (XCXCLh), during early embryogenesis. XCXCLh is expressed in the dorsal vegetal region at the gastrula stage. Both overexpression and knockdown of XCXCLh in the dorsal region inhibited gastrulation. XCXCLh contributed to the attraction of mesendodermal cells and accelerated the reassembly of scratched culture cells. Also, XCXCLh contributed to early endodermal induction. Overexpression of VegTmRNA or high concentrations of calcium ions induced XCXCLh expression. XCXCLh may play roles in both cell movements and differentiation during early Xenopus embryogenesis.
几种趋化因子分子在早期形态发生过程中控制细胞运动。然而，尚不清楚趋化因子分子是否影响细胞命运。在此，我们在非洲爪蟾早期胚胎发育过程中鉴定并表征了CXC型趋化因子配体，即非洲爪蟾CXCLh（XCXCLh）。XCXCLh在原肠胚期的背侧植物区域表达。在背侧区域过表达和敲低XCXCLh均会抑制原肠胚形成。XCXCLh有助于中内胚层细胞的吸引，并加速划痕培养细胞的重新组装。此外，XCXCLh有助于早期内胚层诱导。VegTmRNA的过表达或高浓度钙离子可诱导XCXCLh表达。XCXCLh可能在非洲爪蟾早期胚胎发育过程中的细胞运动和分化中均发挥作用。
