Department of Surgery, University of Calgary, Calgary, Alberta, Canada.
Int J Exp Pathol. 2012 Feb;93(1):11-7. doi: 10.1111/j.1365-2613.2011.00792.x. Epub 2011 Nov 22.
Heterologous IgG antibody (ab) can be produced against Heymann nephritis (HN) antigen (ag) in rabbits by administering it in Freund's complete adjuvant. The developing abs reacted at high titre with rat kidney brush border (BB) regions of the renal proximal tubules in an indirect fluorescence ab test. A single IV injection of the heterologous ab into a susceptible strain of rat resulted in the localization of IgG ab to glomerular fixed ags, producing immune complex glomerular nephritis. The injected ab also reacted with the BB region of the renal proximal tubules. The aim of this experiment was to find out whether heterologous IgG ab against the HN ag can also be produced in recipient rabbits by injecting immune complexes (ICs) composed of a rat kidney tubular preparation [rat kidney fraction 3 (rKF3)] and donor rabbit-derived rabbit anti-rKF3 IgG ab. We found that anti-rKF3 IgG ab--against the BB region of the renal proximal tubules--could be induced in rabbits injected with ICs, and the resulting ab was able to initiate passive HN in rats. This was the first time a pathogenic IgG ab was produced against HN ag in rabbits without the use of adjuvant. Ab responses in recipient rabbits were achieved by ab information transfer. Recipient rabbits injected with the IC produced the same class of immunoglobulin with the same specificity against the target ag rKF3, as was present in the innoculum, namely rabbit anti-rKF3 IgG ab.
用弗氏完全佐剂给兔子注射 Heymann 肾炎（HN）抗原（ag），可以产生针对 HN 抗原的异源 IgG 抗体（ab）。在间接荧光抗体试验中，正在发育的 ab 以高滴度与大鼠肾脏近端小管的刷状缘（BB）区域反应。将异源 ab 单次静脉注射到易感大鼠中，导致 IgG ab 定位到肾小球固定的 ags，产生免疫复合物性肾小球肾炎。注射的 ab 也与肾脏近端小管的 BB 区域反应。本实验的目的是确定用由大鼠肾脏制剂[大鼠肾 3 部分（rKF3）]和供体兔源性兔抗 rKF3 IgG ab 组成的免疫复合物（ICs）注射是否也能在受体兔中产生针对 HN ag 的异源 IgG ab。我们发现，用 ICs 注射的兔子可以诱导针对肾脏近端小管 BB 区域的抗 rKF3 IgG ab，并且由此产生的 ab 能够引发大鼠的被动 HN。这是首次在不使用佐剂的情况下在兔子中产生针对 HN ag 的致病性 IgG ab。受体兔中的 ab 反应通过 ab 信息传递实现。用 IC 注射的受体兔产生了与免疫原相同特异性的同种免疫球蛋白，即兔抗 rKF3 IgG ab。
