Nemoto Y, Yamamoto T, Takada S, Matsui Y, Obinata M
Department of Cell Biology, Tohoku University, Sendai, Japan.
Gene. 1990 Jul 16;91(2):261-5. doi: 10.1016/0378-1119(90)90097-b.
The MER5 cDNA was cloned from RNA preferentially synthesized in murine erythroleukemia (MEL) cells during the early period of MEL cell differentiation. To understand the role of the MER5 gene in the differentiation, we have transferred the MER5 cDNA into MEL cells in both sense and antisense orientations under control of the promoter of the human metallothionein gene. Only in the transformants with the antisense MER5 cDNA, did their elevated expression inhibit differentiation. The result suggests that the MER5 gene product may promote early events in the differentiation of MEL cells.
MER5 cDNA是从鼠类红白血病（MEL）细胞分化早期优先合成的RNA中克隆得到的。为了解MER5基因在分化过程中的作用，我们已将MER5 cDNA以正义和反义方向在人金属硫蛋白基因启动子的控制下转入MEL细胞。只有在带有反义MER5 cDNA的转化体中，其表达升高才会抑制分化。结果表明，MER5基因产物可能促进MEL细胞分化的早期事件。
