Kume T, Watanabe T, Sanokawo R, Chida D, Nakamura T, Oishi M
Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku 113, Tokyo, Japan.
J Biol Chem. 1996 Nov 29;271(48):30916-21. doi: 10.1074/jbc.271.48.30916.
We have cloned cDNA for protein tyrosine phosphatase beta2, which had been implicated in erythroid differentiation of mouse erythroleukemia cells. Expression of cDNA constructs, in which beta2 cDNA is placed under the control of mouse metallothionein-I promoter, by ZnCl2 converted a significant portion (20 to 38%) of the cells to erythroid-like cells, which is 25-50% of the erythroid differentiation efficiency observed by conventional erythroid-inducing agents. Furthermore, introduction and expression of altered protein tyrosine phosphatase beta2 cDNA constructs designed to produce the enzyme lacking the phosphatase activity inhibited erythroid differentiation by 100-20%, depending upon the concentration of erythroid-inducing agents employed. These results strongly suggest that protein tyrosine phosphatase beta2 is involved in triggering erythroid differentiation in mouse erythroleukemia cells.
我们已经克隆了蛋白酪氨酸磷酸酶β2的cDNA，该酶与小鼠红白血病细胞的红系分化有关。将β2 cDNA置于小鼠金属硫蛋白-I启动子控制下的cDNA构建体，通过氯化锌处理可使相当一部分（20%至38%）的细胞转变为类红细胞，这是传统红系诱导剂所观察到的红系分化效率的25%至50%。此外，设计用于产生缺乏磷酸酶活性的酶的改变的蛋白酪氨酸磷酸酶β2 cDNA构建体的导入和表达，根据所使用的红系诱导剂的浓度，可使红系分化受到100%至20%的抑制。这些结果有力地表明，蛋白酪氨酸磷酸酶β2参与触发小鼠红白血病细胞的红系分化。
