Advanced Medical and Dental Institute, Universiti Sains Malaysia, Lot 1-8, PersiaranSeksyen 4/1, Bandar Putra Bertam, Kepala Batas 13200, Penang, Malaysia.
Lipids Health Dis. 2011 Nov 21;10:216. doi: 10.1186/1476-511X-10-216.
Oxidized low density lipoprotein plays an important role in development of foam cells in atherosclerosis. The study was focused on regulation of primary human monocyte growth and CD11b expression in presence of Nigella sativa oil.
Primary human monocytes were isolated from whole blood and grown at 37°C and 5% CO₂ saturation for five days prior to treatment with Nigella sativa oil. The cells were plated and washed before treatment with ox-LDL (10 μg/ml) as positive control and combined treatment of ox-LDL (10 μg/ml) and (140 ng/ml) Nigella sativa oil. The growth progression was monitored every 24 hours for 3 days.
Macrophages showed reduced growth in comparison to monocytes 24 hours after treatment with Nigella sativa oil. The mean cell diameter was significantly different between untreated and treated condition in monocytes and macrophages (p < 0.001). Similarly, intracellular lipid accumulation was hindered in combined treatment with Nigella sativa oil. This was further supported by cell surface expression analysis, where CD11b was markedly reduced in cells treated with combination oxLDL and Nigella sativa oil compared to oxLDL alone. More cells differentiated into macrophage-like cells when monocytes were supplemented with oxidized LDL alone.
The finding provides preliminary evidence on regulation of cell growth and differentiation in monocyte and monocyte-derived macrophages by Nigella sativa oil. Further investigations need to be conducted to explain its mechanism in human monocyte.
氧化型低密度脂蛋白在动脉粥样硬化中泡沫细胞的形成中起着重要作用。本研究专注于研究黑种草籽油对人原代单核细胞生长和 CD11b 表达的调节作用。
从全血中分离出人原代单核细胞，在 37°C 和 5%CO₂饱和度下培养五天，然后用黑种草籽油处理。在将 ox-LDL（10μg/ml）作为阳性对照和 ox-LDL（10μg/ml）与黑种草籽油（140ng/ml）联合处理之前，将细胞铺板并洗涤。在 3 天内每 24 小时监测生长进展。
与未处理组相比，用黑种草籽油处理 24 小时后巨噬细胞的生长速度明显减慢。单核细胞和巨噬细胞的未处理组和处理组之间的平均细胞直径有显著差异（p<0.001）。同样，用黑种草籽油联合处理可阻碍细胞内脂质积累。这一点通过细胞表面表达分析得到了进一步的证实，与单独用 oxLDL 处理的细胞相比，用 oxLDL 和黑种草籽油联合处理的细胞中 CD11b 的表达明显减少。当单核细胞仅补充氧化型 LDL 时，更多的细胞分化为巨噬细胞样细胞。
该研究结果为黑种草籽油对单核细胞和单核细胞来源的巨噬细胞的细胞生长和分化的调节作用提供了初步证据。需要进一步的研究来解释其在人单核细胞中的作用机制。
