Prince Sultan Military College of Health Sciences, Dhahran, Saudi Arabia.
King Fahd Specialist Hospital, Dammam, Saudi Arabia.
Drug Metab Pers Ther. 2022 Apr 11;37(3):315-321. doi: 10.1515/dmpt-2021-0229. eCollection 2022 Sep 1.
Breast cancer is the most commonly diagnosed invasive non-skin malignancy in women worldwide, and it is the leading cause of cancer-related deaths in them. Linn. seed oil has been found to be effective in cancer treatment as well as having anti-cancer properties in some other types of cancers. The study looked into the synergistic cytotoxic effects of Linn. seed oil and doxorubicin in the treatment of human breast cancer cells (MCF-7).
Linn. seed oil was used to evaluate its effect on human breast cancer cells, either alone or in conjunction with doxorubicin. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) tests were used to examine cell proliferation and cell viability, while phase-contrast inverted microscopy was used to examine cellular morphology. Furthermore, the role of seed oil in decreasing cell tumorigenicity features was highlighted by testing the cancer cell migration using the wound healing assay.
Results showed that higher concentrations (50 μg/mL) of Linn. seed oil changed the breast cancer cell morphology and decreased the cell proliferation and viability. Breast cancer cells treated with black seed oil decreased cell movement after 24 hours compared to the untreated cell in the wound healing assay. Whereas, only the higher concentration of doxorubicin (0.5-2.5 μg/mL) reduced cell proliferation and cell viability. Moreover, the combination treatment of 50 μg/mL of black seed oil with different concentrations of doxorubicin caused a significant cell proliferation reduction and decreased cell viability. The activity was seen optimum at lower concentration (0.1 µg/mL) of doxorubicin.
There was decreased cell proliferation and cell viability when seed oil was used alone or in conjunction with doxorubicin in Breast cancer cells (MCF-7) revealing potential opportunities in the field of cancer treatment.
乳腺癌是全球女性最常见的侵袭性非皮肤恶性肿瘤，也是导致女性癌症相关死亡的主要原因。亚麻籽油已被发现可有效治疗癌症，并对其他类型癌症具有抗癌特性。本研究探讨了亚麻籽油与多柔比星联合治疗人乳腺癌细胞（MCF-7）的协同细胞毒性作用。
单独或联合多柔比星使用亚麻籽油评估其对人乳腺癌细胞的作用。3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）试验用于检测细胞增殖和细胞活力，而相差倒置显微镜用于观察细胞形态。此外，通过划痕愈合试验检测癌细胞迁移，突出了种子油在降低细胞致瘤性特征中的作用。
结果表明，较高浓度（50μg/mL）的亚麻籽油改变乳腺癌细胞形态，降低细胞增殖和活力。与未经处理的细胞相比，在用黑种草籽油处理的乳腺癌细胞中，划痕愈合试验 24 小时后细胞迁移减少。而只有较高浓度的多柔比星（0.5-2.5μg/mL）才能降低细胞增殖和活力。此外，50μg/mL 的黑种草籽油与不同浓度的多柔比星联合治疗可显著降低细胞增殖并降低细胞活力。该活性在较低浓度（0.1μg/mL）的多柔比星下表现最佳。
单独或联合使用亚麻籽油或多柔比星均可降低乳腺癌细胞（MCF-7）的细胞增殖和活力，这为癌症治疗领域提供了潜在的机会。
