Synergistic anti-cancer effects of seed oil and conventional cytotoxic agent against human breast cancer.

OBJECTIVES

Breast cancer is the most commonly diagnosed invasive non-skin malignancy in women worldwide, and it is the leading cause of cancer-related deaths in them. Linn. seed oil has been found to be effective in cancer treatment as well as having anti-cancer properties in some other types of cancers. The study looked into the synergistic cytotoxic effects of Linn. seed oil and doxorubicin in the treatment of human breast cancer cells (MCF-7).

METHODS

Linn. seed oil was used to evaluate its effect on human breast cancer cells, either alone or in conjunction with doxorubicin. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) tests were used to examine cell proliferation and cell viability, while phase-contrast inverted microscopy was used to examine cellular morphology. Furthermore, the role of seed oil in decreasing cell tumorigenicity features was highlighted by testing the cancer cell migration using the wound healing assay.

RESULTS

Results showed that higher concentrations (50 μg/mL) of Linn. seed oil changed the breast cancer cell morphology and decreased the cell proliferation and viability. Breast cancer cells treated with black seed oil decreased cell movement after 24 hours compared to the untreated cell in the wound healing assay. Whereas, only the higher concentration of doxorubicin (0.5-2.5 μg/mL) reduced cell proliferation and cell viability. Moreover, the combination treatment of 50 μg/mL of black seed oil with different concentrations of doxorubicin caused a significant cell proliferation reduction and decreased cell viability. The activity was seen optimum at lower concentration (0.1 µg/mL) of doxorubicin.

CONCLUSIONS

There was decreased cell proliferation and cell viability when seed oil was used alone or in conjunction with doxorubicin in Breast cancer cells (MCF-7) revealing potential opportunities in the field of cancer treatment.