Laboratory of Veterinary Internal Medicine, Graduate School of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo, Japan.
Ultrasound Med Biol. 2012 Jan;38(1):109-18. doi: 10.1016/j.ultrasmedbio.2011.09.017. Epub 2011 Nov 21.
Ultrasound targeted microbubble destruction has succeeded in delivering drugs and genes. This study was designed to explore characteristics of ultrasound targeted microbubble destruction using short-pulsed diagnostic ultrasound. Canine thyroid adenocarcinoma cells were exposed to short-pulsed diagnostic ultrasound in the presence of cis-diamminedichloroplatinum (II) (cisplatin) and ultrasound contrast agent Sonazoid(®) microbubbles. The cytotoxic effect of cisplatin was enhanced by short-pulsed diagnostic ultrasound and microbubbles. Incubation time with microbubbles influenced the cytotoxic effect of cisplatin. However, exposure duration did not affect the cytotoxic effect of cisplatin. Therefore, short-pulsed diagnostic ultrasound may activate microbubbles near cells and deliver cisplatin into cells. In addition, activation of microbubbles may be concluded in a short time. Our results suggest that short exposure duration could be potentially sufficient to induce efficient drug delivery by ultrasound targeted microbubble destruction using short-pulsed diagnostic ultrasound.
超声靶向微泡破坏技术已成功将药物和基因递送至病灶部位。本研究旨在探讨短脉冲超声在超声靶向微泡破坏中的作用特点。应用顺铂（cisplatin）和超声造影剂声诺维（Sonazoid(®)）微泡，对犬甲状腺腺癌细胞进行短脉冲超声辐照。结果显示，超声联合微泡可增强顺铂的细胞毒性作用，且微泡孵育时间影响顺铂的细胞毒性作用，但辐照时间对顺铂的细胞毒性作用无影响。因此，短脉冲超声可能通过激活细胞附近的微泡，将顺铂递送至细胞内。此外，微泡的激活可能在短时间内完成。本研究结果提示，在超声靶向微泡破坏中，短时间的辐照可能足以实现有效的药物传递。
