Kumari M, Dutt N H
Department of Studies in Zoology, University of Mysore, Manasagangotri, India.
Folia Morphol (Praha). 1990;38(2):118-27.
The pituitary gland of Puntius sarana is of the leptobasic type and has three subdivisions--a proximal pars distalis (PPD), a rostral pars distalis (RPD) and a pars intermedia (PI) arranged vertically one below the other. The gonadotrophs (GTH cells) of the PPD were identified by their PAS, ATh, AF, aniline blue and alcian blue positivity and by their immunoreactivity to ovine anti-LH beta-subunit, anti-salmon GTH and anti-silver carp GTH. The testes of Puntius sarana have been classified as the "unrestricted type". The morphological, histological and histometric findings indicate that the testing passes through four distinct stages--preparatory, fully mature, spawning and spent. Spermatogenesis begins in January and continues up to July. The spawning season lasts from late July until October. Cyclical changes in the gonadotrophs correlate well with changes in the testes. The histometric data show that the GTH cells attain maximum values (size and number) during the breeding season. The peak values for testicular weight, lobule diameter, the gonadosomatic index (GSI) and the sperm concentration in the testes, and the number, size and amount of secretory material in the cytoplasm of the gonadotrophs, coincide with the time of maximum environmental temperature and the longest days.
萨氏无须魮的脑垂体属于瘦基型，有三个细分部分——近端远侧部（PPD）、吻端远侧部（RPD）和中间部（PI），它们自上而下垂直排列。PPD的促性腺激素细胞（GTH细胞）通过其对PAS、ATh、AF、苯胺蓝和阿尔辛蓝呈阳性反应以及对羊抗LHβ亚基、抗鲑鱼GTH和抗鲤鱼GTH的免疫反应性来鉴定。萨氏无须魮的睾丸已被归类为“无限制型”。形态学、组织学和组织测量学结果表明，睾丸经历四个不同阶段——准备期、完全成熟期、产卵期和耗尽期。精子发生始于1月并持续至7月。产卵季节从7月下旬持续到10月。促性腺激素细胞的周期性变化与睾丸的变化密切相关。组织测量数据表明，GTH细胞在繁殖季节达到最大值（大小和数量）。睾丸重量、小叶直径、性腺体指数（GSI）和睾丸中的精子浓度的峰值，以及促性腺激素细胞细胞质中分泌物质的数量、大小和含量，与最高环境温度和最长日照时间相吻合。
