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本文引用的文献

1
The occurrence of 'bulbs', a complex configuration of the vacuolar membrane, is affected by mutations of vacuolar SNARE and phospholipase in Arabidopsis.液泡“灯泡”结构是液泡膜的一种复杂的形态，其发生会受到拟南芥液泡 SNARE 和磷脂酶突变的影响。
Plant J. 2011 Oct;68(1):64-73. doi: 10.1111/j.1365-313X.2011.04665.x. Epub 2011 Jul 14.
2
Vacuolar/pre-vacuolar compartment Qa-SNAREs VAM3/SYP22 and PEP12/SYP21 have interchangeable functions in Arabidopsis.液泡/前液泡隔室 Qa-SNAREs VAM3/SYP22 和 PEP12/SYP21 在拟南芥中具有可互换的功能。
Plant J. 2010 Dec;64(5):864-73. doi: 10.1111/j.1365-313X.2010.04372.x. Epub 2010 Nov 10.
3
Life with and without AtTIP1;1, an Arabidopsis aquaporin preferentially localized in the apposing tonoplasts of adjacent vacuoles.有和没有AtTIP1;1（一种优先定位于相邻液泡相对的液泡膜上的拟南芥水通道蛋白）的生活。
Plant Mol Biol. 2009 May;70(1-2):193-209. doi: 10.1007/s11103-009-9465-2. Epub 2009 Feb 20.
4
Advancing cell biology and functional genomics in maize using fluorescent protein-tagged lines.利用荧光蛋白标记系推进玉米细胞生物学和功能基因组学研究
Plant Physiol. 2009 Feb;149(2):601-5. doi: 10.1104/pp.108.130146.
5
New insights into the tonoplast architecture of plant vacuoles and vacuolar dynamics during osmotic stress.植物液泡的液泡膜结构及渗透胁迫期间液泡动态变化的新见解。
BMC Plant Biol. 2005 Aug 4;5:13. doi: 10.1186/1471-2229-5-13.
6
Novel regulation of aquaporins during osmotic stress.渗透胁迫期间水通道蛋白的新型调控
Plant Physiol. 2004 Aug;135(4):2318-29. doi: 10.1104/pp.104.044891. Epub 2004 Aug 6.
7
Geminating pollen has tubular vacuoles, displays highly dynamic vacuole biogenesis, and requires VACUOLESS1 for proper function.双生花粉具有管状液泡，表现出高度动态的液泡生物发生，并且其正常功能需要VACUOLESS1。
Plant Physiol. 2004 Mar;134(3):1227-39. doi: 10.1104/pp.103.037382. Epub 2004 Feb 26.
8
High-throughput viral expression of cDNA-green fluorescent protein fusions reveals novel subcellular addresses and identifies unique proteins that interact with plasmodesmata.cDNA-绿色荧光蛋白融合体的高通量病毒表达揭示了新的亚细胞定位，并鉴定出与胞间连丝相互作用的独特蛋白质。
Plant Cell. 2003 Jul;15(7):1507-23. doi: 10.1105/tpc.013284.
9
Vacuolar membrane dynamics revealed by GFP-AtVam3 fusion protein.通过GFP-AtVam3融合蛋白揭示的液泡膜动力学
Genes Cells. 2002 Jul;7(7):743-53. doi: 10.1046/j.1365-2443.2002.00550.x.
10
A complex and mobile structure forms a distinct subregion within the continuous vacuolar membrane in young cotyledons of Arabidopsis.在拟南芥幼嫩子叶的连续液泡膜内，一个复杂且可移动的结构形成了一个独特的亚区域。
Plant J. 2002 Feb;29(3):245-55. doi: 10.1046/j.0960-7412.2001.01189.x.

“泡囊”膜与其他液泡膜之间的定性差异。

Qualitative difference between "bulb" membranes and other vacuolar membranes.

机构信息

Molecular Membrane Biology Laboratory, RIKEN Advanced Science Institute, Wako, Japan.

出版信息

Plant Signal Behav. 2011 Dec;6(12):1914-7. doi: 10.4161/psb.6.12.18061.

DOI:10.4161/psb.6.12.18061
PMID:22105033
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3337177/
Abstract

"Bulb" is a mobile and complex structure appearing in vacuolar membrane of plant cell. We recently reported new fluorescent marker lines for bulbs and bulb-less mutants. We tried multicolor visualization of vacuolar membrane to show distinct segregation of bulb-positive protein (γTIP or AtVAM3) and bulb-negative protein (AtRab75). Unexpectedly, GFP-AtRab75 resulted to localize in bulb under the condition of co-expression with TagRFP-AtVAM3. The signal intensities of GFP-AtRab75 and TagRFP-AtVAM3 were quantified and compared. The result indicates that TagRFP-AtVAM3 is concentrated in bulb than GFP-AtRab75.

摘要

"Bulb" 是一种出现在植物细胞液泡膜上的移动且复杂的结构。我们最近报道了用于灯泡和无灯泡突变体的新型荧光标记线。我们试图对液泡膜进行多色可视化，以显示灯泡阳性蛋白 (γTIP 或 AtVAM3) 和灯泡阴性蛋白 (AtRab75) 的明显分离。出乎意料的是，在与 TagRFP-AtVAM3 共表达的情况下，GFP-AtRab75 定位于灯泡中。对 GFP-AtRab75 和 TagRFP-AtVAM3 的信号强度进行了量化和比较。结果表明，TagRFP-AtVAM3 比 GFP-AtRab75 更集中在灯泡中。