Singh A K, McArdle C, Ashraf M, Granley K, Mishra U, Gordon B
Department of Veterinary Diagnostic Investigation, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
Forensic Sci Int. 1990 Aug;47(1):1-15. doi: 10.1016/0379-0738(90)90280-c.
Equine plasma and urine samples were analyzed by using a high-performance liquid chromatography (HPLC), enzyme-linked immunosorbent assay (ELISA) and particle concentration fluorescence assay (PCFIA). Although ELISA and PCFIA were rapid, simple and sensitive for the screening of furosemide, they did not give reproducible quantitative results. The HPLC method, which required relatively longer analysis time, provided simple and reproducible quantitative analysis of furosemide in plasma and urine. The performance of the three methods was compared for the quantitation of furosemide in plasma obtained from thoroughbred mares dosed intravenously with furosemide (500 micrograms/kg (n = 7) and 1.0 mg/kg (n = 5)). Although the plasma furosemide profiles determined by ELISA, PCFIA and HPLC were similar, ELISA and PCFIA methods exhibited considerable variation in values. At high furosemide concentrations, the PCFIA method gave better quantitative values than ELISA. However, at trace furosemide concentrations the PCFIA method gave false positive values which were not confirmed by HPLC or ELISA. The pharmacokinetic values obtained from the HPLC data and the pharmacokinetic values obtained previously from the gas chromatographic data were comparable. The data obtained by ELISA and PCFIA were not suitable for the pharmacokinetic calculations.
马血浆和尿液样本采用高效液相色谱法（HPLC）、酶联免疫吸附测定法（ELISA）和颗粒浓度荧光测定法（PCFIA）进行分析。虽然ELISA和PCFIA对速尿的筛查快速、简便且灵敏，但它们无法给出可重复的定量结果。HPLC法分析时间相对较长，可对血浆和尿液中的速尿进行简单且可重复的定量分析。比较了这三种方法对静脉注射速尿（500微克/千克（n = 7）和1.0毫克/千克（n = 5））的纯种母马血浆中速尿的定量性能。虽然ELISA、PCFIA和HPLC测定的血浆速尿图谱相似，但ELISA和PCFIA方法的值存在相当大的差异。在高速尿浓度下，PCFIA方法比ELISA给出更好的定量值。然而，在痕量速尿浓度下，PCFIA方法给出的假阳性值未得到HPLC或ELISA的证实。从HPLC数据获得的药代动力学值与先前从气相色谱数据获得的药代动力学值具有可比性。ELISA和PCFIA获得的数据不适合进行药代动力学计算。
