Woods W E, Wang C J, Houtz P K, Tai H H, Wood T, Weckman T J, Yang J M, Chang S L, Blake J W, Tobin T
Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, School of Pharmacy, University of Kentucky, Lexington 40546-0099.
Res Commun Chem Pathol Pharmacol. 1988 Jul;61(1):111-28.
A one step enzyme-linked immunosorbent assay (ELISA) and a particle concentration fluorescent immunoassay (PCFIA) test for furosemide were evaluated as part of a panel of pre- and post-race tests for illegal medication of racing horses. These tests are very sensitive to furosemide with an I-50 for furosemide of about 20 ng/ml. The test is also rapid; an average pre-race complement of 10 samples can be analyzed in 90 minutes or less. The ELISA test results can be read with an inexpensive spectrophotometer, or even by eye. Both the PCFIA test and the ELISA test readily detect the presence of furosemide in equine blood for up to five hours after administration of the recommended therapeutic dose of this agent. The principal utility of these tests lies in rapid screening of samples for compliance with regulations governing the use of furosemide. Thus these tests can be used pre-race to determine whether horsemen have treated their horses with furosemide, and post-race to perform an initial evaluation of whether certain blood concentrations of furosemide have been exceeded. Pilot trials with these systems in Kentucky and Illinois suggest that these tests are economical and effective, and can form part of an analytical approach to substitute for the detention barn system of monitoring furosemide administration.
作为赛马非法用药赛前和赛后检测项目的一部分，对速尿的一步式酶联免疫吸附测定（ELISA）和颗粒浓度荧光免疫测定（PCFIA）进行了评估。这些检测对速尿非常敏感，速尿的半数抑制浓度（I-50）约为20纳克/毫升。该检测也很快速；平均赛前10个样本的补充检测可在90分钟或更短时间内完成。ELISA检测结果可用一台价格低廉的分光光度计读取，甚至肉眼也可读取。在给予该药物推荐治疗剂量后长达5小时内，PCFIA检测和ELISA检测都能轻易检测到马血中速尿的存在。这些检测的主要用途在于快速筛查样本，以确保符合有关速尿使用的规定。因此，这些检测可在赛前用于确定骑手是否用速尿治疗过他们的马匹，赛后用于初步评估是否超过了速尿的某些血药浓度。在肯塔基州和伊利诺伊州对这些系统进行的试点试验表明，这些检测经济有效，可作为一种分析方法的一部分，用以替代监测速尿给药的隔离厩系统。
