Kamada N, Kuramoto A, Katsuki T, Hinuma Y
Blood. 1979 Jun;53(6):1140-7.
Although chromosome aberrations in T lymphocytes and bone marrow cells have been reported in atomic bomb survivors, the presence of chromosome abnormalities has not been demonstrated in B lymphocytes because of the technical difficulties involved in B-lymphocyte separation. A method for detecting chromosome aberrations in B lymphocytes was established by "stimulation" of B lymphocytes with Epstein-Barr virus (EBV) instead of "separation" of B lymphocytes by rosette formation. The EBV-stimulated lymphocytes were isolated as single colonies in soft agar and transferred to liquid culture for further cell growth. The EBV-stimulated B lymphocytes of two heavily exposed survivors showed 50% and 12.5% chromosome abnormalities 30 yr after exposure to the effects of the atomic bomb. The former patient seemed to have a karyotypically abnormal clone of B lymphocytes in vivo. The method used in this study and the evidence of chromosome aberrations in B lymphocytes for long periods after radiation exposure will be useful and important in elucidating the malignant processes of acute lymphocytic leukemia, B-cell lymphoma, and multiple myeloma among high-risk groups having a history of accidental or therapeutic exposure to radiation or radiomimetic drugs.
尽管在原子弹爆炸幸存者中已报告T淋巴细胞和骨髓细胞存在染色体畸变，但由于分离B淋巴细胞存在技术困难，尚未在B淋巴细胞中证实存在染色体异常。通过用爱泼斯坦-巴尔病毒（EBV）“刺激”B淋巴细胞而非通过形成玫瑰花结“分离”B淋巴细胞，建立了一种检测B淋巴细胞染色体畸变的方法。经EBV刺激的淋巴细胞在软琼脂中分离为单个集落，并转移至液体培养中以促进细胞进一步生长。两名受辐射严重的幸存者经EBV刺激的B淋巴细胞在遭受原子弹爆炸影响30年后显示出50%和12.5%的染色体异常。前一位患者体内似乎存在一个核型异常的B淋巴细胞克隆。本研究中使用的方法以及辐射暴露后长时间B淋巴细胞中存在染色体畸变的证据，对于阐明有意外或治疗性辐射或拟辐射药物暴露史的高危人群中急性淋巴细胞白血病、B细胞淋巴瘤和多发性骨髓瘤的恶性进程将是有用且重要的。
