Yang M, Gao F, Liu H, Yu W H, He G Q, Zhuo F, Qiu G P, Sun S Q
Institute of Neuroscience, Chongqing Medical University, China.
Anat Histol Embryol. 2011 Aug;40(4):299-306. doi: 10.1111/j.1439-0264.2011.01070.x. Epub 2011 Apr 18.
The aquaporins (AQPs) are a family of homologous water channels expressed in many tissues. In this study, the expression and immunolocalization of different AQP subtypes in rat brains were investigated by RT-PCR, immunohistochemistry and immunofluorescence. The data showed that AQP1 was expressed in the subpial processes of astrocytes, choroid plexus and ependyma. AQP3, AQP5 and AQP8 had similar distribution patterns in piriform cortex, choroid plexus, hippocampus and dorsal thalamus. AQP4 and AQP9 were widely expressed in the rat brain and distributed in the subpial processes of astrocytes, ependyma, dorsal thalamus, hippocampus, white matter, suprachiasmatic nucleus (SCN) and supraoptic nucleus. AQP3, AQP4, AQP5, AQP8 and AQP9 were found in the Bergmann glial cells of cerebellum, cochlear nucleus and trapezoid nuclei. The distinct localization of various AQPs in cerebrum and the similarities of distribution patterns within cerebellum, cochlear nucleus and trapezoid nuclei suggest that AQPs may play an important role in maintaining the specific microenvironments of the brain.
水通道蛋白（AQPs）是一类在许多组织中表达的同源性水通道家族。在本研究中，通过逆转录聚合酶链反应（RT-PCR）、免疫组织化学和免疫荧光技术，对大鼠脑中不同AQP亚型的表达及免疫定位进行了研究。数据显示，AQP1在星形胶质细胞的软膜下突起、脉络丛和室管膜中表达。AQP3、AQP5和AQP8在梨状皮质、脉络丛、海马和背侧丘脑具有相似的分布模式。AQP4和AQP9在大鼠脑中广泛表达，分布于星形胶质细胞的软膜下突起、室管膜、背侧丘脑、海马、白质、视交叉上核（SCN）和视上核。在小脑的伯格曼胶质细胞、蜗神经核和梯形核中发现了AQP3、AQP4、AQP5、AQP8和AQP9。各种AQP在大脑中的独特定位以及在小脑、蜗神经核和梯形核内分布模式的相似性表明，AQP可能在维持脑的特定微环境中发挥重要作用。
