Dicer 在哺乳动物中非对称 RISC 加载中可有可无。
Dicer is dispensable for asymmetric RISC loading in mammals.
机构信息
Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan.
出版信息
RNA. 2012 Jan;18(1):24-30. doi: 10.1261/rna.029785.111. Epub 2011 Nov 21.
Abstract
In flies, asymmetric loading of small RNA duplexes into Argonaute2-containing RNA-induced silencing complex (Ago2-RISC) requires Dicer-2/R2D2 heterodimer, which acts as a protein sensor for the thermodynamic stabilities of the ends of small RNA duplexes. However, the mechanism of small RNA asymmetry sensing in mammalian RISC assembly remains obscure. Here, we quantitatively examined RISC assembly and target silencing activity in the presence or absence of Dicer in mammals. Our data show that, unlike the well-characterized fly Ago2-RISC assembly pathway, mammalian Dicer is dispensable for asymmetric RISC loading in vivo and in vitro.
摘要
在果蝇中，小 RNA 双链体不对称加载到含有 Argonaute2 的 RNA 诱导沉默复合物（Ago2-RISC）中需要 Dicer-2/R2D2 异二聚体，该异二聚体作为小 RNA 双链体末端热力学稳定性的蛋白质传感器。然而，哺乳动物 RISC 组装中小 RNA 不对称感应的机制仍然不清楚。在这里，我们定量研究了在哺乳动物中存在或不存在 Dicer 的情况下 RISC 组装和靶基因沉默活性。我们的数据表明，与已充分研究的果蝇 Ago2-RISC 组装途径不同，哺乳动物 Dicer 在体内和体外不对称 RISC 加载中并非必不可少。
相似文献
1
Dicer is dispensable for asymmetric RISC loading in mammals.
RNA. 2012 Jan;18(1):24-30. doi: 10.1261/rna.029785.111. Epub 2011 Nov 21.
2
Precursor microRNA-programmed silencing complex assembly pathways in mammals.
Mol Cell. 2012 May 25;46(4):507-17. doi: 10.1016/j.molcel.2012.03.010. Epub 2012 Apr 12.
3
Molecular requirements for RNA-induced silencing complex assembly in the Drosophila RNA interference pathway.
J Biol Chem. 2005 Nov 25;280(47):39278-83. doi: 10.1074/jbc.M509202200. Epub 2005 Sep 22.
4
Native gel analysis for mammalian microRNPs assembled from pre-microRNAs.
Methods Mol Biol. 2015;1206:39-51. doi: 10.1007/978-1-4939-1369-5_4.
5
Defining fundamental steps in the assembly of the Drosophila RNAi enzyme complex.
Nature. 2015 May 28;521(7553):533-6. doi: 10.1038/nature14254. Epub 2015 Mar 30.
6
Viral RNAi suppressor reversibly binds siRNA to outcompete Dicer and RISC via multiple turnover.
J Mol Biol. 2011 Apr 29;408(2):262-76. doi: 10.1016/j.jmb.2011.02.038. Epub 2011 Feb 24.
7
[Components and assembly of RNA-induced silencing complex].
Yi Chuan. 2006 Jun;28(6):761-6.
8
Structure of the Dicer-2-R2D2 heterodimer bound to a small RNA duplex.
Nature. 2022 Jul;607(7918):393-398. doi: 10.1038/s41586-022-04790-2. Epub 2022 Jun 29.
9
TAF11 Assembles the RISC Loading Complex to Enhance RNAi Efficiency.
Mol Cell. 2015 Sep 3;59(5):807-18. doi: 10.1016/j.molcel.2015.07.006. Epub 2015 Aug 6.
10
A human, ATP-independent, RISC assembly machine fueled by pre-miRNA.
Genes Dev. 2005 Dec 15;19(24):2979-90. doi: 10.1101/gad.1384005.
引用本文的文献
1
Regulation of microRNA expression by the adaptor protein GRB2.
Sci Rep. 2023 Jun 16;13(1):9784. doi: 10.1038/s41598-023-36996-3.
2
XRN2 suppresses aberrant entry of tRNA trailers into argonaute in humans and Arabidopsis.
PLoS Genet. 2023 May 5;19(5):e1010755. doi: 10.1371/journal.pgen.1010755. eCollection 2023 May.
3
Utilizing functional cell-free extracts to dissect ribonucleoprotein complex biology at single-molecule resolution.
Wiley Interdiscip Rev RNA. 2023 Sep-Oct;14(5):e1787. doi: 10.1002/wrna.1787. Epub 2023 Apr 12.
4
One locus, several functional RNAs-emerging roles of the mechanisms responsible for the sequence variability of microRNAs.
Biol Futur. 2023 Jun;74(1-2):17-28. doi: 10.1007/s42977-023-00154-7. Epub 2023 Feb 27.
5
The molecular mechanism of microRNA duplex selectivity of Arabidopsis ARGONAUTE10.
Nucleic Acids Res. 2022 Sep 23;50(17):10041-10052. doi: 10.1093/nar/gkac571.
6
The siRNA Off-Target Effect Is Determined by Base-Pairing Stabilities of Two Different Regions with Opposite Effects.
Genes (Basel). 2022 Feb 9;13(2):319. doi: 10.3390/genes13020319.
7
siRNA Seed Region Is Divided into Two Functionally Different Domains in RNA Interference in Response to 2'-OMe Modifications.
ACS Omega. 2022 Jan 4;7(2):2398-2410. doi: 10.1021/acsomega.1c06455. eCollection 2022 Jan 18.
8
Circulating exosomal miRNAs and cancer early diagnosis.
Clin Transl Oncol. 2022 Mar;24(3):393-406. doi: 10.1007/s12094-021-02706-6. Epub 2021 Sep 15.
9
microRNA strand selection: Unwinding the rules.
Wiley Interdiscip Rev RNA. 2021 May;12(3):e1627. doi: 10.1002/wrna.1627. Epub 2020 Sep 20.
10
MicroRNAs Responding to Space Radiation.
Int J Mol Sci. 2020 Sep 9;21(18):6603. doi: 10.3390/ijms21186603.
本文引用的文献
1
Recognition of the pre-miRNA structure by Drosophila Dicer-1.
Nat Struct Mol Biol. 2011 Sep 18;18(10):1153-8. doi: 10.1038/nsmb.2125.
2
siRNA repositioning for guide strand selection by human Dicer complexes.
Mol Cell. 2011 Jul 8;43(1):110-21. doi: 10.1016/j.molcel.2011.05.028.
3
Crystal structure of the MID-PIWI lobe of a eukaryotic Argonaute protein.
Proc Natl Acad Sci U S A. 2011 Jun 28;108(26):10466-71. doi: 10.1073/pnas.1103946108. Epub 2011 Jun 6.
4
Structure of C3PO and mechanism of human RISC activation.
Nat Struct Mol Biol. 2011 Jun;18(6):650-7. doi: 10.1038/nsmb.2032. Epub 2011 May 8.
5
Native gel analysis for RISC assembly.
Methods Mol Biol. 2011;725:91-105. doi: 10.1007/978-1-61779-046-1_7.
6
Phosphate and R2D2 restrict the substrate specificity of Dicer-2, an ATP-driven ribonuclease.
Mol Cell. 2011 Apr 22;42(2):172-84. doi: 10.1016/j.molcel.2011.03.002. Epub 2011 Mar 17.
7
A role for human Dicer in pre-RISC loading of siRNAs.
Nucleic Acids Res. 2011 Mar;39(4):1510-25. doi: 10.1093/nar/gkq846. Epub 2010 Oct 23.
8
Conserved vertebrate mir-451 provides a platform for Dicer-independent, Ago2-mediated microRNA biogenesis.
Proc Natl Acad Sci U S A. 2010 Aug 24;107(34):15163-8. doi: 10.1073/pnas.1006432107. Epub 2010 Aug 10.
9
A direct role for Hsp90 in pre-RISC formation in Drosophila.
Nat Struct Mol Biol. 2010 Aug;17(8):1024-6. doi: 10.1038/nsmb.1875. Epub 2010 Jul 18.
10
In vitro assembly of plant RNA-induced silencing complexes facilitated by molecular chaperone HSP90.
Mol Cell. 2010 Jul 30;39(2):282-91. doi: 10.1016/j.molcel.2010.05.014. Epub 2010 Jun 3.
Zotero 插件