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TAF11 Assembles the RISC Loading Complex to Enhance RNAi Efficiency.TAF11组装RNA诱导沉默复合体以提高RNA干扰效率。
Mol Cell. 2015 Sep 3;59(5):807-18. doi: 10.1016/j.molcel.2015.07.006. Epub 2015 Aug 6.
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Dicer-2 and R2D2 coordinately bind siRNA to promote assembly of the siRISC complexes.Dicer-2和R2D2协同结合小干扰RNA（siRNA）以促进小干扰RNA诱导沉默复合体（siRISC）的组装。
RNA. 2006 Aug;12(8):1514-20. doi: 10.1261/rna.101606. Epub 2006 Jun 14.
3
Defining fundamental steps in the assembly of the Drosophila RNAi enzyme complex.定义果蝇 RNAi 酶复合物组装的基本步骤。
Nature. 2015 May 28;521(7553):533-6. doi: 10.1038/nature14254. Epub 2015 Mar 30.
4
Molecular requirements for RNA-induced silencing complex assembly in the Drosophila RNA interference pathway.果蝇RNA干扰途径中RNA诱导沉默复合体组装的分子要求。
J Biol Chem. 2005 Nov 25;280(47):39278-83. doi: 10.1074/jbc.M509202200. Epub 2005 Sep 22.
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R2D2, a bridge between the initiation and effector steps of the Drosophila RNAi pathway.R2D2，果蝇RNA干扰途径起始步骤与效应步骤之间的桥梁。
Science. 2003 Sep 26;301(5641):1921-5. doi: 10.1126/science.1088710.
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Roles of R2D2, a cytoplasmic D2 body component, in the endogenous siRNA pathway in Drosophila.R2D2 在果蝇内源性 siRNA 通路中的作用，作为细胞质 D2 体成分。
Mol Cell. 2013 Feb 21;49(4):680-91. doi: 10.1016/j.molcel.2012.12.024. Epub 2013 Jan 31.
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R2D2 organizes small regulatory RNA pathways in Drosophila.R2D2 组织果蝇中的小调控 RNA 通路。
Mol Cell Biol. 2011 Feb;31(4):884-96. doi: 10.1128/MCB.01141-10. Epub 2010 Dec 6.
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C3PO, an endoribonuclease that promotes RNAi by facilitating RISC activation.C3PO，一种通过促进RNA诱导沉默复合体（RISC）激活来促进RNA干扰（RNAi）的核糖核酸酶。
Science. 2009 Aug 7;325(5941):750-3. doi: 10.1126/science.1176325.
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The RNAi pathway initiated by Dicer-2 in Drosophila.果蝇中由Dicer-2启动的RNA干扰途径。
Cold Spring Harb Symp Quant Biol. 2006;71:39-44. doi: 10.1101/sqb.2006.71.008.
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Loqs-PD and R2D2 define independent pathways for RISC generation in Drosophila.Loqs-PD 和 R2D2 在果蝇中定义了 RISC 产生的独立途径。
Nucleic Acids Res. 2011 May;39(9):3836-51. doi: 10.1093/nar/gkq1324. Epub 2011 Jan 17.
引用本文的文献
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Molecular mechanisms of biomolecular condensate formation in Drosophila melanogaster siRNA biogenesis.果蝇中参与小干扰RNA生物合成的生物分子凝聚物形成的分子机制
Nucleic Acids Res. 2025 Jul 19;53(14). doi: 10.1093/nar/gkaf664.
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Invading viral DNA triggers dsRNA synthesis by RNA polymerase II to activate antiviral RNA interference in Drosophila.入侵病毒的 DNA 通过 RNA 聚合酶 II 触发 dsRNA 的合成，从而激活果蝇中的抗病毒 RNA 干扰。
Cell Rep. 2022 Jun 21;39(12):110976. doi: 10.1016/j.celrep.2022.110976.
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RNAi for Western Corn Rootworm Management: Lessons Learned, Challenges, and Future Directions.用于西方玉米根虫治理的RNA干扰：经验教训、挑战及未来方向
Insects. 2022 Jan 5;13(1):57. doi: 10.3390/insects13010057.
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Evaluation of , , and Mosquitoes Competence to Infection.评价 、 、 和 蚊子感染 的能力。
Viruses. 2021 Apr 25;13(5):755. doi: 10.3390/v13050755.
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The effector mechanism of siRNA spherical nucleic acids.siRNA 球形核酸的效应机制。
Proc Natl Acad Sci U S A. 2020 Jan 21;117(3):1312-1320. doi: 10.1073/pnas.1915907117. Epub 2020 Jan 3.
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Distinct gene-selective roles for a network of core promoter factors in neural stem cell identity.核心启动子因子网络在神经干细胞特性中具有不同的基因选择性作用。
Biol Open. 2019 Apr 4;8(4):bio042168. doi: 10.1242/bio.042168.
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Mosquito Innate Immunity.蚊子的先天免疫
Insects. 2018 Aug 8;9(3):95. doi: 10.3390/insects9030095.
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Defense Mechanisms against Viral Infection in : RNAi and Non-RNAi.抗病毒感染的防御机制：RNAi 和非 RNAi。
Viruses. 2018 May 1;10(5):230. doi: 10.3390/v10050230.
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Implementing the sterile insect technique with RNA interference - a review.利用RNA干扰实施昆虫不育技术——综述
Entomol Exp Appl. 2017 Sep;164(3):155-175. doi: 10.1111/eea.12575. Epub 2017 Jun 23.
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Molecular basis for asymmetry sensing of siRNAs by the Drosophila Loqs-PD/Dcr-2 complex in RNA interference.果蝇Loqs-PD/Dcr-2复合物在RNA干扰中对小干扰RNA不对称感应的分子基础。
Nucleic Acids Res. 2017 Dec 1;45(21):12536-12550. doi: 10.1093/nar/gkx886.
本文引用的文献
1
Defining fundamental steps in the assembly of the Drosophila RNAi enzyme complex.定义果蝇 RNAi 酶复合物组装的基本步骤。
Nature. 2015 May 28;521(7553):533-6. doi: 10.1038/nature14254. Epub 2015 Mar 30.
2
Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9.利用 CRISPR/Cas9 在果蝇细胞系中进行诱变和同源重组。
Biol Open. 2014 Jan 15;3(1):42-9. doi: 10.1242/bio.20137120.
3
Roles of R2D2, a cytoplasmic D2 body component, in the endogenous siRNA pathway in Drosophila.R2D2 在果蝇内源性 siRNA 通路中的作用，作为细胞质 D2 体成分。
Mol Cell. 2013 Feb 21;49(4):680-91. doi: 10.1016/j.molcel.2012.12.024. Epub 2013 Jan 31.
4
Chromatin-associated RNA interference components contribute to transcriptional regulation in Drosophila.染色质相关 RNA 干扰成分有助于果蝇的转录调控。
Nature. 2011 Nov 6;480(7377):391-5. doi: 10.1038/nature10492.
5
Structure of C3PO and mechanism of human RISC activation.C3PO 的结构和人类 RISCs 激活的机制。
Nat Struct Mol Biol. 2011 Jun;18(6):650-7. doi: 10.1038/nsmb.2032. Epub 2011 May 8.
6
A direct role for Hsp90 in pre-RISC formation in Drosophila.Hsp90 在果蝇前核糖核蛋白体形成中的直接作用。
Nat Struct Mol Biol. 2010 Aug;17(8):1024-6. doi: 10.1038/nsmb.1875. Epub 2010 Jul 18.
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In vitro assembly of plant RNA-induced silencing complexes facilitated by molecular chaperone HSP90.分子伴侣 HSP90 促进植物 RNA 诱导沉默复合物的体外组装。
Mol Cell. 2010 Jul 30;39(2):282-91. doi: 10.1016/j.molcel.2010.05.014. Epub 2010 Jun 3.
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Hsc70/Hsp90 chaperone machinery mediates ATP-dependent RISC loading of small RNA duplexes.Hsc70/Hsp90 伴侣蛋白机器介导 ATP 依赖的小 RNA 双链体的 RISC 加载。
Mol Cell. 2010 Jul 30;39(2):292-9. doi: 10.1016/j.molcel.2010.05.015. Epub 2010 Jun 3.
9
Biochemical principles of small RNA pathways.小 RNA 通路的生化原理。
Annu Rev Biochem. 2010;79:295-319. doi: 10.1146/annurev.biochem.052208.151733.
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Molecular mechanisms that funnel RNA precursors into endogenous small-interfering RNA and microRNA biogenesis pathways in Drosophila.果蝇中引导 RNA 前体进入内源性小干扰 RNA 和 microRNA 生物发生途径的分子机制。
RNA. 2010 Mar;16(3):506-15. doi: 10.1261/rna.1952110. Epub 2010 Jan 19.
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