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临床研究中的胆汁采样、处理与分析

Bile sampling, processing and analysis in clinical studies.

作者信息

Strasberg S M, Harvey P R, Hofmann A F

机构信息

Mount Sinai Research Institute, Toronto, Canada.

出版信息

Hepatology. 1990 Sep;12(3 Pt 2):176S-180S; discussion 180S-182S.

PMID:2210646
Abstract

Obtaining a proper bile sample for investigative purposes is of utmost importance to obtain valid results. Bile can be collected by direct aspiration of the gallbladder, by duodenal intubation or by T-tube drainage. The optimal method of collection depends on the investigative question, as well as on the resources available to the investigator. The procedures for obtaining, processing and analyzing human bile (gallbladder and hepatic) are summarized, pointing out the disadvantages and pitfalls that may occur.

摘要

为了获得有效的结果,获取合适的胆汁样本用于研究目的至关重要。胆汁可以通过直接穿刺胆囊、十二指肠插管或T管引流来收集。最佳的收集方法取决于研究问题以及研究者可利用的资源。本文总结了获取、处理和分析人体胆汁(胆囊胆汁和肝胆汁)的程序,指出了可能出现的缺点和陷阱。

相似文献

1
Bile sampling, processing and analysis in clinical studies.临床研究中的胆汁采样、处理与分析
Hepatology. 1990 Sep;12(3 Pt 2):176S-180S; discussion 180S-182S.
2
Hepatic bile versus gallbladder bile: a comparison of protein and lipid concentration and composition in cholesterol gallstone patients.肝胆汁与胆囊胆汁:胆固醇结石患者蛋白质、脂质浓度及成分的比较
Hepatology. 1998 Jul;28(1):11-6. doi: 10.1002/hep.510280103.
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Nasogastric intubation technique for bile sampling in the baboon (Papio ursinus).狒狒(山魈)胆汁采样的鼻胃插管技术。
Lab Anim Sci. 1979 Apr;29(2):237-9.
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Evaluation and clinical application of the Enterotest for the determination of human biliary porphyrin composition.用于测定人体胆汁卟啉成分的肠检法的评估及临床应用
Eur J Clin Chem Clin Biochem. 1995 Jul;33(7):453-62.
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[Comparative characteristics of lipid components of gallbladder and liver bile fractions in children with chronic cholecystitis].[慢性胆囊炎患儿胆囊胆汁和肝胆汁组分脂质成分的比较特征]
Eksp Klin Gastroenterol. 2003(4):68-72, 116.
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Cholecystokinin-stimulated peak lipase concentration in duodenal drainage fluid: a new pancreatic function test.十二指肠引流液中胆囊收缩素刺激的峰值脂肪酶浓度:一项新的胰腺功能测试。
Am J Gastroenterol. 2002 Jun;97(6):1392-7. doi: 10.1111/j.1572-0241.2002.05675.x.
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A comparison of endoscopic and surgical collection procedures for the analysis of the bacterial flora in duodenal fluid from cats.
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[The use of the Dreimlich probe for obtaining samples in a postoperative study of biliopancreatic function in cholecystectomized patients].[在胆囊切除患者胆胰功能术后研究中使用德赖姆利希探头获取样本]
Nutr Hosp. 1995 Mar-Apr;10(2):123-8.
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Total protein content of human gallbladder bile: relation to cholesterol gallstone disease and effects of treatment with bile acids and aspirin.人胆囊胆汁的总蛋白含量:与胆固醇胆结石疾病的关系以及胆汁酸和阿司匹林治疗的效果
Eur J Surg. 1996 Jun;162(6):463-9.
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The effect of dihydroxydibutylether on bile lipid composition in patients with gallstones.
Int J Clin Pharmacol Ther Toxicol. 1983 Jan;21(1):37-40.

引用本文的文献

1
Comparative proteomic analysis of gallbladder bile proteins related to cholesterol gallstones.胆囊胆石症相关胆汗蛋白质的比较蛋白质组学分析
PLoS One. 2013;8(1):e54489. doi: 10.1371/journal.pone.0054489. Epub 2013 Jan 17.
2
Osteopontin and integrin are involved in cholesterol gallstone formation.骨桥蛋白和整合素参与胆固醇结石的形成。
Med Sci Monit. 2012 Jan;18(1):BR16-23. doi: 10.12659/msm.882194.
3
A novel method for the determination of biliary clearance in humans.一种测定人体胆汁清除率的新方法。
AAPS J. 2004 Nov 23;6(4):e33. doi: 10.1208/aapsj060433.
4
Biliary lipid composition in idiopathic bile acid malabsorption.特发性胆汁酸吸收不良中的胆汁脂质成分
Gut. 1998 Dec;43(6):812-6. doi: 10.1136/gut.43.6.812.
5
Human gallbladder mucosal function: effects on intraluminal fluid and lipid composition in health and disease.人类胆囊黏膜功能:对健康和疾病状态下管腔内液体及脂质成分的影响。
Dig Dis Sci. 1998 Feb;43(2):335-43. doi: 10.1023/a:1018858406560.
6
The effects of dietary phospholipids enriched with phosphatidylethanolamine on bile and red cell membrane lipids in humans.富含磷脂酰乙醇胺的膳食磷脂对人体胆汁和红细胞膜脂质的影响。
Lipids. 1996 Mar;31(3):295-303. doi: 10.1007/BF02529876.
7
Pharmacokinetics and clinical study of cefotetan in bile: prophylactic use in biliary tract surgery.头孢替坦在胆汁中的药代动力学及临床研究:在胆道手术中的预防性应用
Pharm World Sci. 1993 Apr 23;15(2):79-82. doi: 10.1007/BF01874087.
8
Effect of cholecystectomy on gallbladder bile composition.胆囊切除术对胆囊胆汁成分的影响。
Dig Dis Sci. 1992 Aug;37(8):1232-5. doi: 10.1007/BF01296565.