Nelson D R, Buxton T B, Luu Q N, Rissing J P
VA Medical Center, Infectious Disease Laboratory, Augusta, GA 30910.
Infection. 1990 Jul-Aug;18(4):246-8. doi: 10.1007/BF01643399.
We report a model of chronic Pseudomonas aeruginosa osteomyelitis in the rat that was reproducible, simple and inexpensive. No promoting agent was required to cause infection. Infected animals yielded consistent pseudomonal colony counts (log): 4.98 +/- 0.32 (SD)/g cortical tibia (n = 16). The 95% confidence interval of the mean was 4.83-5.14. The inoculum required to infect 50% of challenged rats (ID50) was log 4.0; the ID 100 was log 6.4. Ceftazidime (50 mg/kg/8 h, subcutaneously), alone and in combination with tobramycin (40 mg/kg/12 h, subcutaneously), produced no significant change in quantitative bacterial count or gross bone pathology when used to treat established disease.
我们报道了一种大鼠慢性铜绿假单胞菌骨髓炎模型，该模型具有可重复性、操作简单且成本低廉的特点。无需促发剂即可引发感染。感染动物的假单胞菌菌落计数（对数）一致：胫骨干皮质骨为4.98±0.32（标准差）/克（n = 16）。均值的95%置信区间为4.83 - 5.14。感染50%受试大鼠所需的接种量（ID50）为对数4.0；ID100为对数6.4。当用于治疗已确诊疾病时，头孢他啶（50毫克/千克/8小时，皮下注射）单独使用或与妥布霉素（40毫克/千克/12小时，皮下注射）联合使用，在细菌定量计数或大体骨病理学方面均未产生显著变化。
