Guizar-Sicairos Manuel, Diaz Ana, Holler Mirko, Lucas Miriam S, Menzel Andreas, Wepf Roger A, Bunk Oliver
Swiss Light Source, Paul Scherrer Institut, 5232 Villigen PSI, Switzerland.
Opt Express. 2011 Oct 24;19(22):21345-57. doi: 10.1364/OE.19.021345.
Coherent diffractive imaging provides accurate phase projections that can be tomographically combined to yield detailed quantitative 3D reconstructions with a resolution that is not limited by imaging optics. We present robust algorithms for post-processing and alignment of these tomographic phase projections. A simple method to remove undesired constant and linear phase terms on the reconstructions is given. Also, we provide an algorithm for automatic alignment of projections that has good performance even for samples with no fiducial markers. Currently applied to phase projections, this alignment algorithm has proven to be robust and should also be useful for lens-based tomography techniques that pursue nanoscale 3D imaging. Lastly, we provide a method for tomographic reconstruction that works on phase projections that are known modulo 2π, such that the phase unwrapping step is avoided. We demonstrate the performance of these algorithms by 3D imaging of bacteria population in legume root-nodule cells.
相干衍射成像可提供精确的相位投影，这些投影可通过断层扫描组合，以产生具有不受成像光学器件限制的分辨率的详细定量三维重建。我们提出了用于这些断层相位投影的后处理和对齐的稳健算法。给出了一种去除重建中不需要的常数和线性相位项的简单方法。此外，我们提供了一种投影自动对齐算法，即使对于没有基准标记的样本也具有良好的性能。目前应用于相位投影，这种对齐算法已被证明是稳健的，对于追求纳米级三维成像的基于透镜的断层扫描技术也应该是有用的。最后，我们提供了一种用于断层重建的方法，该方法适用于已知模2π的相位投影，从而避免了相位展开步骤。我们通过对豆科植物根瘤细胞中的细菌群体进行三维成像来展示这些算法的性能。
