Federal Research Centre for Cultivated Plants, Institute for Plant Protection in Fruit Crops and Viticulture, Julius Kuehn Institute, Schwabenheimer Str. 101, 69221 Dossenheim, Germany.
Arch Virol. 2012 Feb;157(2):359-62. doi: 10.1007/s00705-011-1176-8. Epub 2011 Nov 23.
The complete nucleotide sequence of an Albanian isolate of grapevine leafroll-associated virus 7 (GLRaV-7-Alb) was determined. The viral genome consists of 16,404 nucleotides and has nine open reading frames (ORFs) that potentially encode proteins, most of which are typical for members of the family Closteroviridae. Only the 25-kDa (ORF8) and 27-kDa (ORF9) proteins had no apparent similarity to other viral proteins in the sequence databases. The genome structure of GLRaV-7-Alb closely resembles that of little cherry virus 1 and cordyline virus 1. In phylogenetic trees constructed with HSP70h sequences, these three viruses cluster together in a clade next to that comprising members of the genus Crinivirus, to which they are more closely related than to the clostero- and ampeloviruses. The molecular properties of these three viruses differ sufficiently from those of members of the three extant genera of the family Closteroviridae to warrant their classification in a novel genus.
葡萄卷叶伴随病毒 7（GLRaV-7）阿尔巴尼亚分离物的全核苷酸序列已被确定。该病毒基因组由 16404 个核苷酸组成，具有九个开放阅读框（ORF），可能编码蛋白质，其中大多数是典型的杆状病毒科成员。只有 25kDa（ORF8）和 27kDa（ORF9）蛋白与序列数据库中的其他病毒蛋白没有明显的相似性。GLRaV-7-Alb 的基因组结构与樱桃李病毒 1 和百合病毒 1 非常相似。在使用 HSP70h 序列构建的系统发育树中，这三种病毒与包含韧皮部病毒属成员的分支聚类在一起，与它们的亲缘关系比与杆状病毒和番茄斑萎病毒更近。这三种病毒的分子特性与现有杆状病毒科三个属的成员有足够的差异，有理由将它们分类为一个新属。
