Institute of Biological Chemistry, M.J. Murdock Metabolomics Laboratory, Washington State University, Pullman, WA 99164-6340, USA.
Planta. 2012 May;235(5):939-54. doi: 10.1007/s00425-011-1551-4. Epub 2011 Nov 23.
The repeated removal of flower, fruit, or vegetative buds is a common treatment to simulate sink limitation. These experiments usually lead to the accumulation of specific proteins, which are degraded during later stages of seed development, and have thus been designated as vegetative storage proteins. We used oligonucleotide microarrays to assess global effects of sink removal on gene expression patterns in soybean leaves and found an induction of the transcript levels of hundreds of genes with putative roles in the responses to biotic and abiotic stresses. In addition, these data sets indicated potential changes in amino acid and phenylpropanoid metabolism. As a response to sink removal we detected an induced accumulation of γ-aminobutyric acid, while proteinogenic amino acid levels decreased. We also observed a shift in phenylpropanoid metabolism with an increase in isoflavone levels, concomitant with a decrease in flavones and flavonols. Taken together, we provide evidence that sink removal leads to an up-regulation of stress responses in distant leaves, which needs to be considered as an unintended consequence of this experimental treatment.
反复去除花、果或营养芽是模拟汇限制的常用处理方法。这些实验通常会导致特定蛋白质的积累，这些蛋白质在种子发育的后期阶段被降解，因此被指定为营养贮藏蛋白。我们使用寡核苷酸微阵列来评估汇去除对大豆叶片基因表达模式的全局影响，发现数百个基因的转录水平被诱导，这些基因可能在应对生物和非生物胁迫方面具有作用。此外，这些数据集还表明氨基酸和苯丙烷代谢可能发生变化。作为对汇去除的响应，我们检测到 γ-氨基丁酸的诱导积累，而蛋白质氨基酸水平下降。我们还观察到苯丙烷代谢的转移，异黄酮水平增加，同时黄酮和黄酮醇水平降低。总之，我们提供的证据表明，汇去除会导致远处叶片的应激反应上调，这需要被视为这种实验处理的意外后果。
