School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, Guangdong, China.
Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, 510275, Guangdong, China.
Metabolomics. 2019 Apr 11;15(4):62. doi: 10.1007/s11306-019-1523-4.
The brown planthopper (BPH, Nilaparvata lugens Stål, Hemiptera: Delphacidae) is one of the most devastating insect pests of the crucially important cereal crop, rice (Oryza sativa L.). Currently, multiple BPH-resistant rice varieties have been cultivated and generalized to control BPH. However, the defence metabolic responses and their modes of action against BPH in different rice cultivars remain uncharacterized.
We used a non-biased metabolomics approach to explore the differences in metabolite profiles in response to BPH infestation in the susceptible TN1 rice cultivar and two resistant cultivars (IR36 and IR56).
The metabolomic detection based on gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) was performed to investigate the content changes of identified metabolites in TN1, IR36 and IR56 rice varieties at various time points (0 h, 24 h, 48 h and 96 h) post BPH feeding. The differentially expressed metabolites were screened and the corresponding metabolic pathways were further enriched.
The results showed that compared to that in TN1, the content changes of most primary metabolites were more stable, but the concentration alterations of some defence-related metabolites were more acute and persistent in IR36 and IR56. Furthermore, the differentially expressed pathways analysis revealed that cyanoamino acids and lipids metabolism was persistently induced in IR36, but changes in thiamine, taurine and hypotaurine metabolism were more significant in IR56 during BPH infestation. Besides, the contents of quercetin and spermidine which were harmful to BPH fitness, were significantly elevated by BPH in TN1 and IR36, and the quercetin level was significantly decreased during BPH feeding in IR56.
The results of the differences in metabolite profiles in response to BPH infestation in different rice cultivars were useful to clarify the metabolic mechanism of rice plants during BPH infestation and to provide new resources to control this insect pest.
褐飞虱（BPH，Nilaparvata lugens Stål，半翅目：飞虱科）是至关重要的粮食作物水稻（Oryza sativa L.）的最具破坏性的昆虫害虫之一。目前，已经培育和推广了多种抗褐飞虱的水稻品种来控制 BPH。然而，不同水稻品种对 BPH 抗性的防御代谢反应及其作用模式仍不清楚。
我们使用无偏代谢组学方法来探讨感虫品种 TN1 以及两个抗虫品种（IR36 和 IR56）在受到 BPH 侵害时代谢产物谱的差异。
基于气相色谱-质谱联用（GC-MS）和液相色谱-质谱联用（LC-MS）的代谢组学检测，研究了 TN1、IR36 和 IR56 水稻品种在 BPH 取食后不同时间点（0 h、24 h、48 h 和 96 h）的鉴定代谢物含量变化。筛选差异表达代谢物，并进一步富集相应的代谢途径。
结果表明，与 TN1 相比，IR36 和 IR56 中大多数初级代谢物的含量变化更稳定，但一些防御相关代谢物的浓度变化更剧烈且更持久。此外，差异表达途径分析表明，在 IR36 中，氰基氨基酸和脂质代谢持续被诱导，而在 IR56 中，硫胺素、牛磺酸和次牛磺酸代谢的变化更为显著。此外，在 TN1 和 IR36 中，BPH 显著增加了对 BPH 适应性有害的槲皮素和腐胺的含量，而在 IR56 中，BPH 取食期间槲皮素的含量显著降低。
不同水稻品种对 BPH 侵害的代谢产物谱差异的研究结果有助于阐明水稻在 BPH 侵害期间的代谢机制，并为控制这种昆虫害虫提供新的资源。
