PG & Research Deparment of Zoology and Biotechnology, Lady Doak College, Madurai 625 002, Tamil Nadu, India.
Oxid Med Cell Longev. 2011;2011:462607. doi: 10.1155/2011/462607. Epub 2011 Nov 9.
The objective of the study is to find out whether the endothelial nitric oxide synthase (eNOS) G894T single-nucleotide polymorphism is associated with type 2 diabetes mellitus in South Indian (Tamil) population. A total number of 260 subjects comprising 100 type 2 diabetic mellitus patients and 160 healthy individuals with no documented history of diabetes were included for the study. DNA was isolated, and eNOS G894T genotyping was performed using the polymerase chain reaction followed by restriction enzyme analysis using Ban II. The genotype distribution in patients and controls were compatible with the Hardy-Weinberg expectations (P > 0.05). Odds ratio indicates that the occurrence of mutant genotype (GT/TT) was 7.2 times (95% CI = 4.09-12.71) more frequent in the cases than in controls. Thus, the present study demonstrates that there is an association of endothelial nitric oxide synthase gene (G894T) polymorphism with diabetes mellitus among South Indians.
本研究旨在探讨内皮型一氧化氮合酶（eNOS）G894T 单核苷酸多态性与南印度（泰米尔）人群 2 型糖尿病的关系。共纳入 260 例受试者，包括 100 例 2 型糖尿病患者和 160 例无糖尿病病史的健康个体。提取 DNA，采用聚合酶链反应（PCR）结合 Ban II 内切酶分析进行 eNOS G894T 基因分型。患者和对照组的基因型分布符合 Hardy-Weinberg 预期（P＞0.05）。比值比表明，与对照组相比，突变基因型（GT/TT）在病例组中的发生频率高 7.2 倍（95%可信区间=4.09-12.71）。因此，本研究表明，内皮型一氧化氮合酶基因（G894T）多态性与南印度人群的糖尿病有关。
