Department of Clinical Pathology, School of Medicine, University Sumatera Utara, Medan, Indonesia.
Department of Internal Medicine, School of Medicine, University Sumatera Utara, Medan, Indonesia.
Med Glas (Zenica). 2020 Aug 1;17(2):316-320. doi: 10.17392/1088-20.
Aim To assess endothelial nitric oxide synthase (eNOS) gene (G894T) polymorphism and nitric oxide (NO) level in hypertensive diabetic Bataknese patients. Methods A hospital-based, case control study (hypertensive and normotensive diabetic patient) was conducted. Genotyping of eNOS gene (G894T) was done using polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP). Nitric oxide was quantified by sandwich enzyme-linked immunosorbent assay (Sandwich ELISA). Results GT polymorphism and T allele were higher in the hypertensive diabetic patients, 37.5% (p=0.6) and 54% (p=0.39), respectively. Nitric oxide level tended to be lower in the hypertensive diabetic patients (88.87µmol/L) comparing to the normotensive (95.42 µmol/L (p=0.54), as well as GT and TT polymorphism type (p=0.75). Conclusion eNOS gene (G894T) polymorphism is not associated with NO level and hypertension in the diabetic Bataknese patients.
评估高血压糖尿病巴塔克患者内皮型一氧化氮合酶（eNOS）基因（G894T）多态性和一氧化氮（NO）水平。
进行了一项基于医院的病例对照研究（高血压和正常血压糖尿病患者）。使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）对 eNOS 基因（G894T）进行基因分型。通过夹心酶联免疫吸附测定（Sandwich ELISA）定量测定一氧化氮。
GT 多态性和 T 等位基因在高血压糖尿病患者中更高，分别为 37.5%（p=0.6）和 54%（p=0.39）。与正常血压组（95.42µmol/L（p=0.54）相比，高血压糖尿病患者的一氧化氮水平较低，GT 和 TT 多态性类型也较低（p=0.75）。
eNOS 基因（G894T）多态性与糖尿病巴塔克患者的 NO 水平和高血压无关。
