University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, Baltimore, Maryland 21201, USA.
J Biomed Opt. 2011 Nov;16(11):116004. doi: 10.1117/1.3644394.
In this paper, fluorescence metal nanoshells (FMNs) were synthesized for target molecule detection on tissue specimens by fluorescence imaging method. FMNs were made with 40 nm silica spherical cores and 10 nm silver shells. Ru(bpy)(3)(2+) complexes were encapsulated in the silica cores for fluorescence properties. Avidin molecules were covalently bound on FMNs and formed avidin-Ag complexes could be site-specially conjugated on bone tissue specimens. Fluorescence intensity and lifetime images were recorded on a time-resolved confocal microscope. Imaging measurements showed that the emissions by avidin-FMN complexes could be distinctly isolated as individuals from the cellular backgrounds on lifetime images even when the tissues were stained with additional organic dyes. This observation demonstrates that the metal nanoprobes can be used for single target molecule detection on tissues during fluorescence imaging measurements.
本文通过荧光成像方法，合成荧光金属纳米壳（FMNs），以用于组织标本上靶分子的检测。FMNs 由 40nm 的二氧化硅球形核和 10nm 的银壳组成。将 Ru(bpy)(3)(2+)配合物包埋在二氧化硅核中以获得荧光特性。亲和素分子通过共价键结合到 FMNs 上，形成的亲和素-Ag 复合物可以在骨组织标本上进行位点特异性结合。通过时间分辨共焦显微镜记录荧光强度和寿命图像。成像测量结果表明，即使组织被额外的有机染料染色，在寿命图像上，通过亲和素-FMN 复合物的发射也可以作为个体从细胞背景中明显分离出来。这一观察结果表明，在荧光成像测量中，金属纳米探针可用于组织上单个靶分子的检测。
