反义抑制烟草中小叶绿体蛋白 CP12：从转录水平的观点。
Antisense suppression of the small chloroplast protein CP12 in tobacco: a transcriptional viewpoint.
机构信息
Department of Biological Sciences, University of Essex, Colchester, UK.
出版信息
Plant Signal Behav. 2011 Dec;6(12):2026-30. doi: 10.4161/psb.6.12.18055.
Abstract
The chloroplast protein CP12 forms a multi-enzyme complex with the Calvin-Benson cycle enzymes phosphoribulokinase (PRK) and NADP-glyceraldehyde-3-phosphate dehydrogenase (GAPDH). PRK and GAPDH are inactivated when present in this complex, a process shown in vitro to be dependent upon oxidized CP12. Recently we reported on the importance of CP12 in vivo to higher plant metabolism using antisense suppression of CP12 in tobacco (Nicotiana tabacum). Our results indicated that while only minor changes in photosynthetic carbon fixation and in PRK and GAPDH activities were observed, striking changes in growth rates and morphology were seen. In this article we present data on the transcriptional changes observed in one of the antisense lines and we discuss the major findings in light of the metabolic phenotype described.
摘要
叶绿体蛋白 CP12 与卡尔文-本森循环酶磷酸核酮糖激酶 (PRK) 和 NADP-甘油醛-3-磷酸脱氢酶 (GAPDH) 形成多酶复合物。当 PRK 和 GAPDH 存在于该复合物中时，它们会失活，体外实验表明这一过程依赖于 CP12 的氧化。最近，我们使用烟草（Nicotiana tabacum）中的 CP12 反义抑制来报道 CP12 在高等植物代谢中的重要性。我们的结果表明，虽然只观察到光合作用碳固定和 PRK 和 GAPDH 活性的微小变化，但在生长速度和形态上却出现了显著的变化。在本文中，我们介绍了反义系之一中观察到的转录变化的数据，并根据所描述的代谢表型讨论了主要发现。
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