Department of Food and Applied Life Sciences, Yamagata University, Tsuruoka, Japan.
Plant Signal Behav. 2011 Dec;6(12):1966-72. doi: 10.4161/psb.6.12.17967.
During seed development, endosperm cells of highly productive cereals, including rice, synthesize disulfide-rich proteins in large amounts and deposit them into storage organelles. Disulfide bond formation involves electron transfer and generates H(2)O(2) as a by-product. To ensure proper development and maturation of seeds, the endosperm cells must supply large amounts of oxidizing equivalents to dithiols in nascent proteins in a controlled manner. This review compares multiple oxidative protein folding systems in yeast, cultured human cells, and rice endosperm. We discuss possible roles of ERO1, other sulfhydryl oxidases, and the protein disulfide isomerase family in the formation of disulfide bonds in storage proteins and the development of protein bodies. Rice prolamins, encoded by a multigene family, are divided into Cys-rich and Cys-depleted subgroups. We discuss the potential importance of disulfide bond formation in the evolution of the prolamin family in japonica rice.
在种子发育过程中，包括水稻在内的高产品种的胚乳细胞大量合成富含二硫键的蛋白质，并将其沉积到储存细胞器中。二硫键的形成涉及电子转移，并产生 H(2)O(2)作为副产物。为了确保种子的正常发育和成熟，胚乳细胞必须以可控的方式将大量的氧化当量供应给新生蛋白质中的二硫醇。本综述比较了酵母、培养的人类细胞和水稻胚乳中的多种氧化蛋白折叠系统。我们讨论了 ERO1、其他巯基氧化酶和蛋白二硫键异构酶家族在储存蛋白中二硫键形成和蛋白体发育中的可能作用。由多基因家族编码的水稻醇溶蛋白分为富含半胱氨酸和半胱氨酸耗尽亚组。我们讨论了二硫键形成在粳稻醇溶蛋白家族进化中的潜在重要性。
