Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Fukuoka 812-8581, Japan.
Plant Sci. 2011 Aug;181(2):125-31. doi: 10.1016/j.plantsci.2011.04.011. Epub 2011 Apr 30.
The esp1 mutant CM21 specifically exhibits reduced levels of cysteine-poor (CysP) prolamin bands with pIs of 6.65, 6.95, 7.10, and 7.35 in rice seed. Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis demonstrated that the bands with pIs 6.65, 6.95, and 7.35 are encoded by different structural genes. These results suggest that the Esp1 locus encodes a regulatory factor involved in the synthesis and/or accumulation of CysP prolamin molecules. Isoelectric focusing (IEF) analysis of CysP prolamins in chromosome substitution lines showed that structural genes for bands with pI values of 6.95, 7.10, and 7.35, which are reduced in esp1 mutant lines, are located as a gene cluster in the 44.2 cM region on chromosome 5.
esp1 突变体 CM21 中，稻米种子中 pI 值为 6.65、6.95、7.10 和 7.35 的半胱氨酸缺乏（CysP）醇溶蛋白带的水平明显降低。基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）分析表明，pI 值为 6.65、6.95 和 7.35 的条带由不同的结构基因编码。这些结果表明 Esp1 基因座编码一种参与 CysP 醇溶蛋白分子合成和/或积累的调节因子。在染色体代换系中对 CysP 醇溶蛋白进行等电聚焦（IEF）分析表明，esp1 突变体系中减少的 pI 值为 6.95、7.10 和 7.35 的条带的结构基因位于 5 号染色体上 44.2 cM 区域的一个基因簇中。
