Biocatalysis and Technical Biology Research Group, Cape Peninsula University of Technology, Bellville Campus, Symphony Way, P.O. Box 1906, Bellville, 7535 Cape Town, South Africa.
Enzyme Microb Technol. 2011 Mar 7;48(3):195-208. doi: 10.1016/j.enzmictec.2010.11.007. Epub 2010 Dec 3.
Many industries are currently pursuing enzymatic approaches for developing green chemistry technologies mainly due to shortcomings of physico-chemical methods, growing environmental concerns, legal restrictions, and increasing scientific knowledge. Laccase-assisted reactions, in particular, are being intensively investigated as they are generally eco-friendly and have wide application potential. Laccases only require oxygen as co-substrate, they release water as the only by-product and have a wide substrate range which can be further extended by use of laccase-mediator systems. Consequently, research covering various applications of laccase has been rapidly increasing in recent years, particularly in the areas of coupling and grafting reactions. This review summarizes the advances that have been made in developing technologies based on laccase-mediated coupling and grafting reactions for potential application in areas such as environmental pollution control, modification of lignocellulose materials, food industry, biosensors, textile industry, pharmaceutical industry, and in organic synthesis.
许多行业目前都在追求酶法来开发绿色化学技术，主要是因为物理化学方法存在缺陷、环境问题日益严重、法律限制以及科学知识不断增加。过氧化物酶辅助反应，特别是，正在被深入研究，因为它们通常是环保的，并且具有广泛的应用潜力。过氧化物酶只需要氧气作为共底物，它们只释放水作为唯一的副产物，并且具有广泛的底物范围，可以通过使用过氧化物酶-介体系统进一步扩展。因此，近年来，涵盖过氧化物酶各种应用的研究迅速增加，特别是在偶联和接枝反应领域。本综述总结了在开发基于过氧化物酶介导的偶联和接枝反应的技术方面的进展，这些技术有可能应用于环境污染控制、木质纤维素材料改性、食品工业、生物传感器、纺织工业、制药工业和有机合成等领域。
