Orthopedic Hospital of Passo Fundo, Av. Sete de Setembro, n. 817, Bairro Centro, Passo Fundo, RS, CEP 99010-121, Brazil.
Knee Surg Sports Traumatol Arthrosc. 2012 Oct;20(10):1895-900. doi: 10.1007/s00167-011-1789-1. Epub 2011 Nov 24.
To assess the mechanical behavior and the histology of collagen fibers after prolotherapy with 12.5% dextrose into rat Achilles tendons and to compare with those of corticosteroid treatment.
Out of 60 adult female Wistar rats (70 tendons), 15 received 12.5% dextrose (group I); 15 were treated with corticosteroid injection (group II); and 15 were given 0.9% saline injection (group III), all into the right Achilles tendon, whereas 13 animals received no injections (group IV). Three doses of each substance (groups I, II, and III) were given at a 5-day interval. Collagen fiber color was quantitatively assessed in three samples from each group and in five samples from the control group using picrosirius red staining under polarized and nonpolarized light. Twelve tendons from each group treated with the test substance and 20 tendons from the control group were submitted to the tensile strength test.
There was no statistical difference across the groups with respect to maximum load at failure (n.s.) and absorbed energy (n.s.). With respect to tendon rupture, there was no difference between the myotendinous and the tendinous regions (n.s.). However, hematoxylin-eosin staining revealed statistical significance in lymphocytic inflammatory infiltrate (P = 0.008) and in parallel fiber orientation (P = 0.003) when comparing groups to the control group, without significance for either neovascularization (n.s.) or the presence of fibroblasts (n.s.). Likewise, there was no significant difference between the percentage of mature (n.s.) and immature (n.s.) fibers.
Dextrose was not deleterious to the tendinous tissue, as it did not change the mechanical and histological properties of Achilles tendons in rats. The data obtained in this study may help clinicians in their daily work as they suggest that injections of 12.5% dextrose caused no harm to the tendons, although the clinical importance in humans still needs to be defined.
评估向大鼠跟腱内注射 12.5%葡萄糖后胶原纤维的机械性能和组织学特性，并与皮质类固醇治疗进行比较。
在 60 只成年雌性 Wistar 大鼠（70 跟腱）中，15 只接受 12.5%葡萄糖（I 组）；15 只接受皮质类固醇注射（II 组）；15 只接受 0.9%生理盐水注射（III 组），均注射至右侧跟腱，而 13 只动物未接受注射（IV 组）。每组均给予 3 次剂量，间隔 5 天。用偏振光和非偏振光下的苦味酸天狼猩红染色法对每组的 3 个样本和对照组的 5 个样本进行胶原纤维颜色的定量评估。每组接受测试物质治疗的 12 跟腱和对照组的 20 跟腱进行拉伸强度测试。
各组间最大破坏负荷（无统计学差异）和吸收能量（无统计学差异）无统计学差异。关于腱-肌连接部和腱部的腱断裂，各组间无差异（无统计学差异）。然而，苏木精-伊红染色显示，与对照组相比，淋巴细胞炎性浸润（P = 0.008）和平行纤维取向（P = 0.003）有统计学意义，但新生血管形成（无统计学差异）和纤维母细胞存在（无统计学差异）无统计学意义。同样，成熟（无统计学差异）和不成熟（无统计学差异）纤维的百分比也无显著差异。
葡萄糖对腱组织没有损害，因为它没有改变大鼠跟腱的机械和组织学特性。本研究获得的数据可能有助于临床医生的日常工作，因为它们表明，尽管在人类中其临床重要性仍有待确定，但 12.5%葡萄糖注射对肌腱无害。
