a Danish Technological Institute , Plastics Technology , Teknologiparken , Aarhus C , 8000 , Denmark.
Biofouling. 1996;10(1-3):17-30. doi: 10.1080/08927019609386268.
A putative polysaccharide adhesin which mediates non-specific attachment of Hyphomonas MHS-3 (MHS-3) to hydrophilic substrata has been isolated and partially characterized. A polysaccharide-enriched portion of the extracellular polymeric substance (EPS(P)) from MHS-3 was separated into four fractions using high performance size exclusion chromatography (HPSEC). Comparison of chromatograms of EPS(P) from MHS-3 and a reduced adhesion strain (MHS-3 rad) suggested that one EPS(P) fraction, which consisted of carbohydrate, served as an adhesin. Adsorption of this fraction to germanium (Ge) was investigated using attenuated total reflection Fourier transform infrared (ATR/FT-IR) spectrometry. Binding curves indicated that the isolated fraction had a relatively high affinity for Ge when ranked against an adhesive protein from Mytilis edulis, mussel adhesive protein (MAP) and an acidic polysaccharide (alginate from Macrocystis pyrifera). Spectral features were used to identify the fraction as a polysaccharide previously reported to adsorb preferentially out of the EPS(P) mixture. Conditioning the Ge substratum with either bovine serum albumin (BSA) or MAP decreased the adsorption of the adhesive polysaccharide significantly. Conditioning Ge with these proteins also decreased adhesion of whole cells.
一种假定的多糖黏附素，介导了噬氢菌 MHS-3（MHS-3）对亲水基质的非特异性黏附，已被分离并部分表征。使用高效体积排阻色谱（HPSEC）将 MHS-3 细胞外聚合物质（EPS(P)）的多糖富集部分分离成四个部分。比较 MHS-3 和减少黏附菌株（MHS-3 rad）的 EPS(P) 的色谱图表明，一种由碳水化合物组成的 EPS(P) 部分作为黏附素。使用衰减全反射傅里叶变换红外（ATR/FT-IR）光谱法研究了该部分对锗（Ge）的吸附。结合曲线表明，与贻贝黏附蛋白（MAP）和酸性多糖（巨藻中的褐藻酸钠）相比，从贻贝 Mytilis edulis 中分离出的这种部分对 Ge 具有相对较高的亲和力。光谱特征表明该部分为多糖，先前报道该多糖优先从 EPS(P)混合物中吸附。用牛血清白蛋白（BSA）或 MAP 预处理 Ge 基底会显著降低黏附多糖的吸附。用这些蛋白质预处理 Ge 也会降低整个细胞的黏附。
