Bhosle N, Suci PA, Baty AM, Weiner RM, Geesey GG
Marine Corrosion and Material Research Division, National Institute of Oceanography, Dona Paula, Goa, 403004, India
J Colloid Interface Sci. 1998 Sep 1;205(1):89-96. doi: 10.1006/jcis.1998.5597.
Hyphomonas MHS-3 (MHS-3) elaborates a diffuse capsular material, primarily composed of polysaccharide, which has been implicated to serve as the holdfast of this prosthecate marine bacterium. A purified polysaccharide (fr2ps) from this capsular material exhibits a relatively large affinity for (Ge), or more precisely for the Ge oxide surface film. In its natural habitat MHS-3 attaches to marine sediments. This suggests that molecular properties of fr2ps have evolved to render it adhesive toward mineral oxides. In order to characterize these molecular interactions, the effect of divalent cations and pH on the adsorption of fr2ps to Ge has been measured using attenuated total internal reflection Fourier transform infrared (ATR/FT-IR) spectroscopy. The effect of adsorption of fr2ps on the Ge oxide film has been investigated using X-ray photoelectron spectroscopy (XPS). The results indicate that divalent cations participate in binding of fr2ps to Ge oxide and that atomic size of the cation is important. Evidence for significant participation of hydrogen bonding to the oxide surface is lacking. Copyright 1998 Academic Press.
海生单毛菌MHS - 3（MHS - 3）能产生一种主要由多糖组成的弥散性荚膜物质，该物质被认为是这种有柄海洋细菌的固着器。从这种荚膜物质中纯化得到的一种多糖（fr2ps）对锗（Ge），或者更确切地说对锗的氧化表面膜具有相对较大的亲和力。在其自然生境中，MHS - 3附着于海洋沉积物。这表明fr2ps的分子特性已经进化，使其对矿物氧化物具有粘附性。为了表征这些分子相互作用，使用衰减全内反射傅里叶变换红外（ATR/FT - IR）光谱法测量了二价阳离子和pH对fr2ps吸附到锗上的影响。使用X射线光电子能谱（XPS）研究了fr2ps吸附对锗氧化膜的影响。结果表明二价阳离子参与了fr2ps与锗氧化物的结合，并且阳离子的原子大小很重要。缺乏氢键与氧化物表面大量参与的证据。版权所有1998年学术出版社。
