Appl Environ Microbiol. 1995 May;61(5):1897-903. doi: 10.1128/aem.61.5.1897-1903.1995.
Hyphomonas strain MHS-3 (MHS-3) is a marine procaryote with a biphasic life cycle and which has prosthecate stages that adhere to submerged substrata. We found that adherent forms produced an exopolysaccharide (EPS) capsule that bound Glycine max lectin, Arachis hypogaea lectin, and Bauhinia purpurea lectin (BPA), each having affinity for N-acetyl-d-galactosamine. It also bound the dye Calcofluor. BPA and Calcofluor were tested for the ability to hinder MHS-3 adhesion to glass surfaces; they reduced attachment by >50 and >85%, respectively. Periodate treatment also reduced attachment (by >80%), but pronase treatment did not. Furthermore, an EPS(sup-) variant, Hyphomonas strain MHS-3 rad, did not attach well to surfaces. These results suggest that the MHS-3 EPS capsule is an adhesin.
海单胞菌 MHS-3 株（MHS-3）是一种具有双相生命周期的海洋原核生物，具有附着于水下基质的附器阶段。我们发现，附着形式产生了一种胞外多糖（EPS）胶囊，该胶囊结合了 Glycine max 凝集素、Arachis hypogaea 凝集素和 Bauhinia purpurea 凝集素（BPA），每种凝集素都对 N-乙酰-d-半乳糖胺具有亲和力。它还结合了染料 Calcofluor。我们测试了 BPA 和 Calcofluor 阻止 MHS-3 附着到玻璃表面的能力；它们分别使附着减少了 >50%和 >85%。过碘酸盐处理也减少了附着（>80%），但溶菌酶处理没有。此外，EPS（sup-）变体海单胞菌 MHS-3 rad 不能很好地附着在表面上。这些结果表明，MHS-3 的 EPS 胶囊是一种黏附素。
