大肠杆菌的小细胞形成突变体：通过使minC基因产物失活来抑制DicB和MinD依赖性的分裂抑制
Minicell-forming mutants of Escherichia coli: suppression of both DicB- and MinD-dependent division inhibition by inactivation of the minC gene product.
作者信息
Labie C, Bouché F, Bouché J P
机构信息
Centre de Recherches de Biochimie et de Génétique cellulaires du Centre National de la Recherche Scientifique, Toulouse, France.
出版信息
J Bacteriol. 1990 Oct;172(10):5852-5. doi: 10.1128/jb.172.10.5852-5855.1990.
Abstract
We have determined the nucleotide sequence of the minB operon of 10 min mutants of Escherichia coli, characterized by impaired inhibition of polar divisions. These mutants were either sensitive or resistant to the division inhibitor DicB. All the mutations were found to lie in minC or minD, confirming the requirement of both gene products in the process of inhibition of polar sites. Mutations conferring resistance to inhibitor DicB were found exclusively in minC. In agreement with de Boer et al. (P. A. J. de Boer, R. E. Crossley, and L. I. Rothfield, Proc. Natl. Acad. Sci. USA 87:1129-1133, 1990), these results provide evidence that, in addition to promoting division inhibition with MinD, protein MinC acts in concert with DicB to inhibit division by a second, MinD-independent process.
摘要
我们已经确定了10个大肠杆菌min突变体的minB操纵子的核苷酸序列，这些突变体的特征是对极性分裂的抑制受损。这些突变体对分裂抑制剂DicB要么敏感要么耐药。所有突变均位于minC或minD中，证实了这两种基因产物在抑制极性位点过程中的必要性。赋予对抑制剂DicB耐药性的突变仅在minC中发现。与德布尔等人（P.A.J.德布尔、R.E.克罗斯利和L.I.罗斯菲尔德，《美国国家科学院院刊》87:1129-1133，1990年）的研究结果一致，这些结果表明，除了与MinD一起促进分裂抑制外，MinC蛋白还与DicB协同作用，通过另一种不依赖MinD的过程抑制分裂。
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Minicell-forming mutants of Escherichia coli: suppression of both DicB- and MinD-dependent division inhibition by inactivation of the minC gene product.大肠杆菌的小细胞形成突变体：通过使minC基因产物失活来抑制DicB和MinD依赖性的分裂抑制
J Bacteriol. 1990 Oct;172(10):5852-5. doi: 10.1128/jb.172.10.5852-5855.1990.
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