Department of Anatomical Sciences, Faculty of medical Sciences, Tarbiat Modares, Tehran, Iran.
Int J Neurosci. 2012 May;122(5):237-47. doi: 10.3109/00207454.2011.642037. Epub 2012 Jan 5.
An in vitro technique was devised to induced autologous adult stem cells into oligodendrocyte-like cells. In this study, a protocol was developed for the induction of bone marrow stromal cells (BMSCs) into oligodendrocyte-like cells. BMSCs were incubated in one of these three pre-inducers: dimethyl sulfoxide (DMSO), β-mercaptoethanol (βME) or biotylated hydroxyanisol (BHA), each followed by retinoic acid (RA) treatment. The percentage of viable cells in BHA-RA preinduced cells was significantly lower than the others. The results showed that the preinduced cells were immunoreactive for nestin and NF-68; among the mentioned protocols, the immunoreactivity yielded by following the DMSO-RA protocol was significantly higher than the others. Moreover, no significant immunoreactivity was observed for preinduced cells to O4, O1, MBP (myelin basic protein), S100, and GFAP (glial fibrillary acidic protein). The cells were immunoreactive to oligo-2. Two phases of induction were done: the first was a combination of basic fibroblast growth factor (bFGF), platelet-derived growth factor (PDGF) and heregulin (HRG), followed by either triiodothyronine (T3) or Forskolin (FSK) as the second phase. The conclusion is that the trans-differentiation of BMSCs by DMSO followed by RA (preinduction stage) then bFGF-PDGF-HRG followed by T3 (10 ng/ml) (induction stage) can be a potential source for oligodendrocyte-like cells preparation.
设计了一种体外技术，将自体成体干细胞诱导为少突胶质细胞样细胞。本研究中，建立了诱导骨髓基质细胞（BMSCs）为少突胶质细胞样细胞的方案。BMSCs 在三种预诱导剂之一中孵育：二甲基亚砜（DMSO）、β-巯基乙醇（βME）或生物素化羟基茴香醚（BHA），随后进行维甲酸（RA）处理。BHA-RA 预诱导细胞中的活细胞百分比明显低于其他细胞。结果表明，预诱导细胞对巢蛋白和 NF-68 呈免疫反应性；在所提到的方案中，DMSO-RA 方案产生的免疫反应性明显高于其他方案。此外，预诱导细胞对 O4、O1、MBP（髓鞘碱性蛋白）、S100 和 GFAP（胶质纤维酸性蛋白）无明显免疫反应性。细胞对少突胶质细胞 2 呈免疫反应性。进行了两个诱导阶段：第一阶段是碱性成纤维细胞生长因子（bFGF）、血小板衍生生长因子（PDGF）和 HRG 的组合，随后是三碘甲状腺素（T3）或 Forskolin（FSK）作为第二阶段。结论是，DMSO 随后 RA（预诱导阶段）然后 bFGF-PDGF-HRG 随后 T3（10ng/ml）（诱导阶段）可以使 BMSCs 发生转分化，成为少突胶质细胞样细胞制备的潜在来源。
